FastQCFastQC Report
Wed 25 May 2016
SRR1781014_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1781014_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences299657
Sequences flagged as poor quality0
Sequence length76
%GC60

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG6911323.064036548453732No Hit
CTTATACACATCTGACGCTGCCGACGAACATAAGGGTGTAGATCTCGGTG14130.47153912640118534No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAACGTG150.00222255970.0058441
TCCGCAC200.00694016952.51314526
GGACCGG200.006953927752.486866
GCCGTTG305.865162E-446.67835226
GTCGGGC305.8796594E-446.65498710
TGGTCGC2350.041.6916949
GATCTCG2400.040.8367441
TCTCGGT2350.040.2161243
AGATCTC2450.040.01001740
GCCGTAT2100.039.9899954
TCGCCGT2200.039.7627752
ATCTCGG2400.039.37828442
GTCGCCG2500.039.1901951
GATGTAT459.172072E-538.8986324
CGTGCGA459.199174E-538.87915810
CGGTGGT2600.037.68287346
CTCGGTG2600.036.3431244
TCGGTGG2700.036.28721245
CGTTGAG400.002413621435.00876228
ATTACGG400.002413621435.00876227
Produced by FastQC (version 0.11.5)