FastQCFastQC Report
Wed 25 May 2016
SRR1781014_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1781014_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences299657
Sequences flagged as poor quality0
Sequence length76
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CATATACACATCTCCGAGCCCACGAGACTTTCGTCAATCTCGTATGCCGT4086613.63759231387887No Hit
CTTATACACATCTCCGAGCCCACGAGACTTTCGTCAATCTCGTATGCCGT61872.0646939667686723No Hit
TCTTATACACATCTCCGAGCCCACGAGACTTTCGTCAATCTCGTATGCCG30051.0028132164441343No Hit
CACTTATACACATCTCCGAGCCCACGAGACTTTCGTCAATCTCGTATGCC22880.7635396469963992No Hit
CAAATACACATCTCCGAGCCCACGAGACTTTCGTCAATCTCGTATGCCGT22460.749523622007829No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG22290.7438504690362647No Hit
CTCTTATACACATCTCCGAGCCCACGAGACTTTCGTCAATCTCGTATGCC8530.28465879322024845No Hit
AAACACATCTCCGAGCCCACGAGACTTTCGTCAATCTCGTATGCCGTCTT6880.22959583790800814RNA PCR Primer, Index 48 (95% over 23bp)
ACTTATACACATCTCCGAGCCCACGAGACTTTCGTCAATCTCGTATGCCG6120.20423350697630957No Hit
TCTTTATACACATCTCCGAGCCCACGAGACTTTCGTCAATCTCGTATGCC5620.18754776294229736No Hit
CAATACACATCTCCGAGCCCACGAGACTTTCGTCAATCTCGTATGCCGTC4440.14816940702202852RNA PCR Primer, Index 48 (95% over 21bp)
CATATACAAATCTCCGAGCCCACGAGACTTTCGTCAATCTCGTATGCCGT3530.11780135288012629No Hit
ATACACATCTCCGAGCCCACGAGACTTTCGTCAATCTCGTATGCCGTCTT3290.1097921957438004RNA PCR Primer, Index 48 (95% over 23bp)
CATAAACACATCTCCGAGCCCACGAGACTTTCGTCAATCTCGTATGCCGT3100.10345161301087576No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGATTG150.002223337369.99966458
CATATAC48400.067.190641
ATATACA48850.066.500042
AGGGGGG60850.064.93806570
GCCGTAT453.8380676E-1062.22192446
TCGTCTA352.9685907E-759.99971431
TGACGAC308.365281E-658.33305445
GACGACT308.365281E-658.33305446
CAAATAC6050.057.2820171
TGCCGTA553.6379788E-1157.27245345
AAGGGGG69650.056.3313969
AATCTCG71050.056.1081136
TATGCCG70400.056.0295643
CCGTCTT69050.056.00987247
CGTCTTC69800.056.00975848
ACATTCG509.786163E-1055.99973327
AATAACG252.3882315E-455.99973336
GCCGTCT69450.055.9896646
CGTATGC71300.055.91137341
GTATGCC70650.055.88083342