FastQCFastQC Report
Wed 25 May 2016
SRR1781013_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1781013_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences115160
Sequences flagged as poor quality0
Sequence length76
%GC80

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG6574157.08666203542897No Hit
CTTATACACATCTGACGCTGCCGACGAACATAAGGGTGTAGATCTCGGTG16041.3928447377561655No Hit
ATACACATCTGACGCTGCCGACGAACATAAGGGTGTAGATCTCGGTGGTC1460.1267801319902744Illumina Single End PCR Primer 1 (95% over 22bp)
TCTTATACACATCTGACGCTGCCGACGAACATAAGGGTGTAGATCTCGGT1400.12156998957971517No Hit
GGTGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG1330.11549149010072941No Hit
CTCTTATACACATCTGACGCTGCCGACGAACATAAGGGTGTAGATCTCGG1310.11375477596387634No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACCATTT150.0022198170.065
CCGGCGA150.0022198170.021
ACCGCGG150.0022198170.030
GTCTAAC150.0022198170.01
GGCTTTC150.0022198170.013
GGGCGAG207.8984056E-570.022
ATCCATT150.0022198170.043
TCGGTGA150.0022198170.060
GTACGGT150.0022198170.011
CGTGTGG252.3819819E-455.9999969
TCTGGCG509.695214E-1055.99999611
TGCCGGC452.6751877E-854.44444719
CTTGTGC200.006936240552.51
TCCATTG200.006936240552.544
TCCATGA200.006936240552.534
CACGGCG200.006936240552.57
GTTTCAA200.006936240552.512
CTTTTAA200.006936240552.524
AACTTTT200.006936240552.522
AGCCAAC200.006936240552.518
Produced by FastQC (version 0.11.5)