##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1781013_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 115160 Sequences flagged as poor quality 0 Sequence length 76 %GC 80 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 28.880279610976032 32.0 27.0 32.0 22.0 32.0 2 29.472221257381037 32.0 32.0 32.0 22.0 32.0 3 29.443122612018062 32.0 32.0 32.0 22.0 32.0 4 28.833067037165684 32.0 32.0 32.0 13.0 32.0 5 29.492054532823897 32.0 32.0 32.0 22.0 32.0 6 33.6638502952414 37.0 37.0 37.0 22.0 37.0 7 33.38557658909343 37.0 37.0 37.0 13.0 37.0 8 33.58463876345954 37.0 37.0 37.0 22.0 37.0 9 33.73367488711358 37.0 37.0 37.0 22.0 37.0 10-11 33.55682962834318 37.0 37.0 37.0 17.5 37.0 12-13 33.37542983674887 37.0 37.0 37.0 17.5 37.0 14-15 34.07320250086836 37.0 37.0 37.0 24.5 37.0 16-17 34.10687738798194 37.0 37.0 37.0 24.5 37.0 18-19 33.66477943730462 37.0 37.0 37.0 22.0 37.0 20-21 34.04497655435915 37.0 37.0 37.0 24.5 37.0 22-23 34.065730288294546 37.0 37.0 37.0 24.5 37.0 24-25 33.69968739145537 37.0 37.0 37.0 22.0 37.0 26-27 34.13947551233067 37.0 37.0 37.0 24.5 37.0 28-29 34.00607415769365 37.0 37.0 37.0 22.0 37.0 30-31 33.77815213615839 37.0 37.0 37.0 22.0 37.0 32-33 33.858948419590135 37.0 37.0 37.0 22.0 37.0 34-35 33.900846648141716 37.0 37.0 37.0 22.0 37.0 36-37 34.43468652309829 37.0 37.0 37.0 29.5 37.0 38-39 34.492011114970474 37.0 37.0 37.0 29.5 37.0 40-41 34.07304619659604 37.0 37.0 37.0 24.5 37.0 42-43 33.98223341437999 37.0 37.0 37.0 22.0 37.0 44-45 33.78360976033345 37.0 37.0 37.0 22.0 37.0 46-47 34.052005904828064 37.0 37.0 37.0 24.5 37.0 48-49 34.441481417158734 37.0 37.0 37.0 29.5 37.0 50-51 34.663550712052796 37.0 37.0 37.0 32.0 37.0 52-53 33.912934178534215 37.0 37.0 37.0 22.0 37.0 54-55 34.02575547064953 37.0 37.0 37.0 24.5 37.0 56-57 33.92597690170198 37.0 37.0 37.0 24.5 37.0 58-59 33.689996526571726 37.0 37.0 37.0 22.0 37.0 60-61 33.430153699201114 37.0 37.0 37.0 17.5 37.0 62-63 33.21104116012504 37.0 37.0 37.0 13.0 37.0 64-65 33.07808700937825 37.0 37.0 37.0 13.0 37.0 66-67 33.07444859326155 37.0 37.0 37.0 13.0 37.0 68-69 33.26251302535603 37.0 37.0 37.0 13.0 37.0 70-71 33.38858110455019 37.0 37.0 37.0 13.0 37.0 72-73 33.65488016672455 37.0 37.0 37.0 22.0 37.0 74-75 33.80445032997569 37.0 37.0 37.0 22.0 37.0 76 34.18536818339701 37.0 37.0 37.0 27.0 37.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 11101 1 0.625372322975938 11101 2 -0.5327438997166993 11101 3 0.39919391149734906 11101 4 -0.45952718014435945 11101 5 0.3521357300858874 11101 6 0.868210370994305 11101 7 1.1869761851777625 11101 8 0.3400896173423007 11101 9 1.0584436376370974 11101 10-11 0.591430159622 11101 12-13 1.0274568268811493 11101 14-15 0.5612952221813075 11101 16-17 -0.0063491674921110075 11101 18-19 0.00138766320212369 11101 20-21 0.32590720965024644 11101 22-23 0.40222942779766413 11101 24-25 0.81247844592599 11101 26-27 0.2661049446840025 11101 28-29 1.441110735748083 11101 30-31 0.1329008287779061 11101 32-33 -0.13942360648963614 11101 34-35 -0.240732718764896 11101 36-37 0.8687115709481787 11101 38-39 0.5094905806309029 11101 40-41 0.06553168519367603 11101 42-43 -0.7445471455568153 11101 44-45 0.06536579306935408 11101 46-47 0.937883225173529 11101 48-49 0.4333822105978129 11101 50-51 0.8368265419108525 11101 52-53 -0.28305117973688 11101 54-55 -0.1382475131375429 11101 56-57 -0.07821191219529311 11101 58-59 0.1836025369557035 11101 60-61 0.5044017091676665 11101 62-63 0.05081173921447402 11101 64-65 0.6223927298617653 11101 66-67 -1.061191183970955 11101 68-69 0.072780541877556 11101 70-71 0.36889708413967526 11101 72-73 0.16530065573395092 11101 74-75 -0.18037074939070408 11101 76 -0.08534890755348101 11102 1 0.5454018051944729 11102 2 -0.3932561532637777 11102 3 0.4898517334984547 11102 4 0.5038043105503149 11102 5 -0.1991817565549816 11102 6 0.060397583082014705 11102 7 2.060202645468898 11102 8 0.21257902217001856 11102 9 0.3230465490061789 11102 10-11 0.21129380436126155 11102 12-13 0.32541149797015123 11102 14-15 0.11989113152348807 11102 16-17 -0.3312063629960704 11102 18-19 0.6538660121978879 11102 20-21 -0.7588541686434738 11102 22-23 -0.13886141428820054 11102 24-25 0.042716146312947956 11102 26-27 -0.9375066270867691 11102 28-29 -0.20694528046713856 11102 30-31 0.28215456012119233 11102 32-33 -0.7179200133442478 11102 34-35 -0.202221570801008 11102 36-37 -0.6975312888270224 11102 38-39 0.04680401346671914 11102 40-41 -0.13513166472340288 11102 42-43 0.28318456625449784 11102 44-45 -0.18219271070805831 11102 46-47 -0.640521049748159 11102 48-49 -0.33711714447865404 11102 50-51 -0.7688487851575019 11102 52-53 -0.6513946475828405 11102 54-55 -0.575137139081896 11102 56-57 -0.19973393172726617 11102 58-59 -0.9683230204424973 11102 60-61 1.114315105150709 11102 62-63 -0.1670802821601356 11102 64-65 0.10820441219085808 11102 66-67 -0.16815635810031182 11102 68-69 0.18094343113497047 11102 70-71 -0.7170620092821025 11102 72-73 -0.34612370394911096 11102 74-75 -0.8074206848983465 11102 76 0.8354176301284824 11103 1 1.016618340081422 11103 2 0.8336823957721471 11103 3 -0.2384046127354189 11103 4 1.2456547752472211 11103 5 1.5459978800104217 11103 6 1.109364152647835 11103 7 0.6942711709231943 11103 8 1.1561218159000788 11103 9 0.8285258110397464 11103 10-11 0.7468049709585785 11103 12-13 1.1165059632219183 11103 14-15 1.3312935451730823 11103 16-17 0.8308133346041515 11103 18-19 1.261994740176796 11103 20-21 1.2688889302606796 11103 22-23 1.5160144353442035 11103 24-25 0.3720960880524373 11103 26-27 0.5968948155543714 11103 28-29 1.3617882470678921 11103 30-31 1.0518174814695058 11103 32-33 0.6961457378001512 11103 34-35 0.8271861140373886 11103 36-37 0.8734910443675972 11103 38-39 1.271523114590316 11103 40-41 1.2456423677360249 11103 42-43 1.1490466830529513 11103 44-45 1.5700614160779054 11103 46-47 2.025590754940424 11103 48-49 0.6949954925028976 11103 50-51 0.6736536423636466 11103 52-53 1.466236091770746 11103 54-55 1.1269713364348135 11103 56-57 0.5930944772060087 11103 58-59 1.3796447975627757 11103 60-61 1.5355940620101975 11103 62-63 1.1484974678745488 11103 64-65 0.8661458742521688 11103 66-67 1.4254766007011952 11103 68-69 0.5268305167224341 11103 70-71 1.7756554272555576 11103 72-73 1.591176892527514 11103 74-75 1.0030939495863294 11103 76 0.6376093349419065 11104 1 0.9281178545771915 11104 2 0.9030401430325163 11104 3 1.0700986470787015 11104 4 1.5778783846243876 11104 5 0.7514355273956355 11104 6 0.727064249748679 11104 7 0.3688446207775371 11104 8 2.0027024789601384 11104 9 1.1625527218456853 11104 10-11 1.0137629401637298 11104 12-13 1.4303497695750877 11104 14-15 0.6631010080666897 11104 16-17 0.8539788221891129 11104 18-19 0.34522403688924186 11104 20-21 1.5004050906157929 11104 22-23 0.8179287091685907 11104 24-25 0.7896297265598591 11104 26-27 0.945209422295946 11104 28-29 0.6510794108908868 11104 30-31 0.39943851073847725 11104 32-33 1.5475120854211823 11104 34-35 1.7360500341372642 11104 36-37 1.3271600691976744 11104 38-39 0.9789027788988207 11104 40-41 1.0130164834247495 11104 42-43 0.7091104921804288 11104 44-45 1.0153381534894734 11104 46-47 1.5570098144493727 11104 48-49 0.2739939666324531 11104 50-51 0.5706573876820045 11104 52-53 1.53379053760235 11104 54-55 0.9495542189427937 11104 56-57 0.7076734756801386 11104 58-59 0.8032819178291106 11104 60-61 1.077278068113685 11104 62-63 1.8884752733954215 11104 64-65 1.9785747825612319 11104 66-67 2.165176975233031 11104 68-69 1.5698323200238562 11104 70-71 1.3817034228166634 11104 72-73 1.0057281479027367 11104 74-75 1.5567768459658495 11104 76 0.8354176301284824 11105 1 0.4538249963180867 11105 2 0.1251060908368622 11105 3 0.4987593753174906 11105 4 -0.14817256083583885 11105 5 1.1099290420072947 11105 6 0.657365859375183 11105 7 1.194911193679559 11105 8 0.9405052606447768 11105 9 0.18068055431950825 11105 10-11 0.03673527959176681 11105 12-13 -0.0018271330659516138 11105 14-15 0.04136323653989393 11105 16-17 -0.1236739360543595 11105 18-19 0.3939816552671189 11105 20-21 0.44219286995630824 11105 22-23 0.300113425530526 11105 24-25 0.5401376184179654 11105 26-27 0.3502727134351886 11105 28-29 0.4303417956385047 11105 30-31 0.7062051930325808 11105 32-33 0.9217893162384954 11105 34-35 0.7515212015071597 11105 36-37 0.320440269228925 11105 38-39 -0.2506643409636595 11105 40-41 0.6342072944889736 11105 42-43 0.29881200804228314 11105 44-45 0.00783698143133904 11105 46-47 0.5263018913363879 11105 48-49 0.7282055621942689 11105 50-51 0.8913324931672335 11105 52-53 1.1192726441814997 11105 54-55 0.8457880054719382 11105 56-57 0.51209604170608 11105 58-59 1.087154397904122 11105 60-61 0.41654982932637097 11105 62-63 1.2535323135579475 11105 64-65 1.1668854561039694 11105 66-67 1.4921811946423063 11105 68-69 1.0629562456372312 11105 70-71 1.7137865608069731 11105 72-73 1.3614087229926 11105 74-75 1.4801242439967908 11105 76 0.062015536051276854 11106 1 0.8653884585144596 11106 2 0.2800838534095611 11106 3 0.7461079897547123 11106 4 -0.4593254859128173 11106 5 -0.3955447862513459 11106 6 0.0747452640963644 11106 7 0.37435012628304776 11106 8 0.9594926024169297 11106 9 0.6086655012917959 11106 10-11 0.1536528300536233 11106 12-13 0.5283644509231067 11106 14-15 0.10687811851047258 11106 16-17 -0.09630479476116705 11106 18-19 0.7841629758281883 11106 20-21 -0.11237435549698915 11106 22-23 0.90676754800743 11106 24-25 0.3202436738404728 11106 26-27 0.04656077364730038 11106 28-29 0.08643142957624406 11106 30-31 0.12674915471578885 11106 32-33 0.3378857924615559 11106 34-35 1.0506145987018272 11106 36-37 0.3966462220171607 11106 38-39 0.08058779725050158 11106 40-41 -1.0172637968555307 11106 42-43 0.3213060210426235 11106 44-45 0.2575803957317149 11106 46-47 -0.6557028982633426 11106 48-49 0.06236567167082541 11106 50-51 -0.4811444141198038 11106 52-53 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24-25 1.0563327040471435 21612 26-27 0.356974128178301 21612 28-29 0.7746269780659105 21612 30-31 0.4880369130623663 21612 32-33 0.12385558578429112 21612 34-35 0.25729186420262096 21612 36-37 -0.23093724380160552 21612 38-39 0.08356871934907417 21612 40-41 0.32156768168182737 21612 42-43 0.29825355681369103 21612 44-45 0.568987390962242 21612 46-47 -0.060909575528839355 21612 48-49 1.0973418242940411 21612 50-51 -0.9415975142787829 21612 52-53 -0.2692232605094844 21612 54-55 0.5336137686886175 21612 56-57 -0.18466494116808008 21612 58-59 0.1932601313367428 21612 60-61 0.30104350021244386 21612 62-63 -0.20774840125962157 21612 64-65 0.5403031776229597 21612 66-67 -0.13384263261011142 21612 68-69 0.6576137288836961 21612 70-71 -0.47822758662415055 21612 72-73 -0.10983103873487465 21612 74-75 -1.827210082138727 21612 76 -0.2622584773515513 >>END_MODULE >>Per sequence quality scores pass #Quality Count 9 1.0 10 2.0 11 14.0 12 35.0 13 73.0 14 164.0 15 259.0 16 504.0 17 759.0 18 855.0 19 964.0 20 945.0 21 995.0 22 1227.0 23 1259.0 24 1498.0 25 1627.0 26 1901.0 27 2006.0 28 2083.0 29 2462.0 30 2861.0 31 3282.0 32 4263.0 33 6015.0 34 9572.0 35 17933.0 36 51601.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 77.63980548801668 7.207363667940257 4.027440083362278 11.125390760680792 2 69.8718171396811 7.807343592593879 14.023691249522354 8.29714801820266 3 68.9136853073984 8.770406391108024 13.721778395276138 8.594129906217436 4 68.22247308093088 8.961444946161862 12.763112191733242 10.052969781174019 5 67.5460229246266 11.444077804793332 15.38381382424453 5.626085446335534 6 73.16516151441472 14.34265369920111 7.02327196943383 5.46891281695033 7 71.84091698506427 10.930010420284821 7.795241403265023 9.433831191385899 8 72.20823202500868 13.679228898923238 7.262070163251129 6.850468912816949 9 70.8310177144842 6.944251476207016 7.1908648836401525 15.033865925668636 10-11 72.14180270927405 10.080757207363668 10.3959708232025 7.381469260159777 12-13 71.4692601597777 8.815560958666204 10.148489058700939 9.566689822855158 14-15 73.02101424105592 9.408648836401529 8.225512330670371 9.344824591872177 16-17 72.52474817645016 9.449027440083363 8.596734977422717 9.429489406043764 18-19 70.5076547669703 9.478364320015285 10.245143586581797 9.76883732643261 20-21 72.2770987560515 9.457047956060178 9.010702733213208 9.255150554675119 22-23 72.49525645534554 9.221363969901828 8.642152201569168 9.641227373183451 24-25 71.54283729210995 10.45942073038343 8.83321030005645 9.16453167745017 26-27 72.47265416364512 10.306959455645249 8.789748622395145 8.43063775831448 28-29 71.46355973788545 10.284825930059363 9.420230066744542 8.831384265310643 30-31 71.30691194608022 10.494293605711606 10.095627703371724 8.103166744836452 32-33 71.75235704638528 11.364198951651778 8.993472794625369 7.889971207337569 34-35 73.49205039454884 9.7028180819324 9.171686289156312 7.633445234362446 36-37 72.82048832179555 9.199246063111815 10.133415559937115 7.846850055155522 38-39 72.72557501216038 9.216263636995345 10.44663331248697 7.6115280383573065 40-41 72.57760522521583 9.831587541473413 9.736046693418105 7.854760539892647 42-43 71.74183967241447 9.618265585715465 10.91358324901972 7.726311492850351 44-45 70.96082288016743 8.652572803098392 11.163937771641201 9.222666545092983 46-47 71.9200743333015 8.585235808504912 10.286825811717916 9.207864046475681 48-49 71.96075026050713 8.49209795067732 11.422368878082667 8.124782910732893 50-51 72.77613754775963 8.754341785342133 10.364709968739145 8.104810698159083 52-53 70.57068922099012 9.295843138617043 10.942696619456578 9.190771020936271 54-55 71.29415749070826 9.379016290944458 9.960401542255722 9.366424676091563 56-57 71.29235632283725 9.222866769717076 9.605832189936955 9.878944717508729 58-59 70.76502257728377 9.936609934004863 10.513633205974296 8.78473428273706 60-61 70.31087183049671 9.26667245571379 10.8475164987843 9.57493921500521 62-63 70.64391472363336 10.22491424601624 10.252268681342539 8.87890234900786 64-65 70.9035255296978 10.178881556095867 10.368617575547065 8.548975338659256 66-67 70.91890342917185 11.284832275375784 8.621992202085812 9.174272093366563 68-69 70.93297903749631 11.86977891245072 8.309453099220201 8.88778895083277 70-71 71.30203335518803 11.610054318328853 8.109539792192228 8.978372534290887 72-73 71.62634566196363 11.650316792384825 8.060292604124598 8.663044941526945 74-75 72.41359842266635 11.066089932337945 8.015217447950596 8.505094197045105 76 73.83849701644213 9.814906497815532 8.13855521102049 8.20804127472184 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.5 8 1.0 9 1.0 10 0.5 11 1.0 12 2.0 13 2.5 14 9.0 15 11.0 16 12.0 17 17.0 18 23.0 19 29.5 20 40.0 21 50.0 22 56.5 23 83.0 24 111.0 25 119.0 26 160.5 27 270.5 28 329.5 29 320.0 30 379.0 31 491.5 32 620.0 33 695.0 34 777.0 35 932.0 36 1097.0 37 1189.0 38 1333.0 39 1623.5 40 1884.5 41 2124.5 42 2250.0 43 2603.5 44 3366.5 45 3086.5 46 2397.0 47 2456.0 48 2416.0 49 2132.0 50 1947.0 51 1834.5 52 1668.5 53 1508.5 54 1402.0 55 1317.0 56 1070.0 57 850.5 58 793.0 59 713.0 60 596.0 61 519.0 62 479.0 63 426.5 64 359.0 65 333.5 66 306.5 67 290.0 68 276.5 69 232.5 70 188.0 71 174.0 72 163.0 73 146.0 74 152.0 75 164.0 76 158.0 77 135.0 78 125.0 79 132.0 80 123.0 81 122.5 82 131.0 83 131.0 84 138.0 85 157.5 86 170.0 87 170.0 88 199.5 89 236.5 90 262.0 91 310.5 92 341.0 93 358.5 94 449.5 95 632.0 96 741.0 97 918.0 98 1656.5 99 34431.0 100 66644.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.010420284821118444 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.002605071205279611 20-21 0.0021708926710663425 22-23 0.0030392497394928796 24-25 0.013025356026398054 26-27 0.012591177492184786 28-29 0.016932962834317472 30-31 0.022577283779089963 32-33 0.02301146231330323 34-35 0.024748176450156305 36-37 0.026919069121222643 38-39 0.027787426189649186 40-41 0.022577283779089963 42-43 0.012591177492184786 44-45 0.0030392497394928796 46-47 0.002605071205279611 48-49 0.0 50-51 0.0 52-53 8.68357068426537E-4 54-55 0.003473428273706148 56-57 0.005210142410559222 58-59 0.0 60-61 0.0 62-63 0.004341785342132685 64-65 0.0 66-67 8.68357068426537E-4 68-69 0.001736714136853074 70-71 0.0047759638763459535 72-73 0.018669676971170546 74-75 0.025182354984369573 76 0.025182354984369573 >>END_MODULE >>Sequence Length Distribution pass #Length Count 76 115160.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 22.681486627301144 #Duplication Level Percentage of deduplicated Percentage of total 1 73.00535987748852 16.558700937825634 2 9.17687595712098 4.162903786036818 3 6.1715160796324655 4.199374782910733 4 4.356814701378254 3.9527613754775968 5 2.741194486983155 3.1087183049670024 6 1.7266462480857578 2.349774227162209 7 0.9954058192955589 1.5804098645362974 8 0.55895865237366 1.0142410559221953 9 0.31776416539050534 0.6486627301146232 >10 0.8843797856049005 2.8230288294546715 >50 0.04211332312404288 0.6443209447724905 >100 0.015313935681470138 0.47759638763459533 >500 0.0 0.0 >1k 0.0038284839203675345 1.3928447377561655 >5k 0.0 0.0 >10k+ 0.0038284839203675345 57.08666203542897 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG 65741 57.08666203542897 No Hit CTTATACACATCTGACGCTGCCGACGAACATAAGGGTGTAGATCTCGGTG 1604 1.3928447377561655 No Hit ATACACATCTGACGCTGCCGACGAACATAAGGGTGTAGATCTCGGTGGTC 146 0.1267801319902744 Illumina Single End PCR Primer 1 (95% over 22bp) TCTTATACACATCTGACGCTGCCGACGAACATAAGGGTGTAGATCTCGGT 140 0.12156998957971517 No Hit GGTGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG 133 0.11549149010072941 No Hit CTCTTATACACATCTGACGCTGCCGACGAACATAAGGGTGTAGATCTCGG 131 0.11375477596387634 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.03126085446335533 0.0 2 0.0 0.0 0.0 0.03473428273706148 0.0 3 0.0 0.0 0.0 0.035602639805488015 0.0 4 0.0 0.0 0.0 0.039076068079194164 0.0 5 0.0 0.0 0.0 0.048627995831886074 0.0 6 0.0 0.0 0.0 0.048627995831886074 0.0 7 0.0 0.0 0.0 0.048627995831886074 0.0 8 0.0 0.0 0.0 0.048627995831886074 0.0 9 0.0 0.0 0.0 0.05470649531087183 0.0 10 0.0 0.0 0.0 0.05470649531087183 0.0 11 0.0 0.0 0.0 0.05470649531087183 0.0 12 0.0 0.0 0.0 0.055574852379298365 0.0 13 0.0 0.0 0.0 0.05644320944772491 0.0 14 0.0 0.0 0.0 0.06078499478985759 0.0 15 0.0 0.0 0.0 0.06252170892671066 0.0 16 0.0 0.0 0.0 0.06252170892671066 0.0 17 0.0 0.0 0.0 0.0633900659951372 0.0 18 0.0 0.0 0.0 0.06946856547412296 0.0 19 0.0 0.0 0.0 0.07381035081625564 0.0 20 0.0 0.0 0.0 0.07815213615838833 0.0 21 0.0 0.0 0.0 0.0798888502952414 0.0 22 0.0 0.0 0.0 0.10420284821118445 0.0 23 0.0 0.0 0.0 0.11549149010072941 0.0 24 0.0 0.0 0.0 0.12504341785342132 0.0 25 0.0 0.0 0.0 0.1363320597429663 0.0 26 0.0 0.0 0.0 0.1380687738798194 0.0 27 0.0 0.0 0.0 0.14848905870093781 0.0 28 0.0 0.0 0.0 0.17019798541160125 0.0 29 0.0 0.0 0.0 0.1927752691906912 0.0 30 0.0 0.0 0.0 0.21708926710663426 0.0 31 0.0 0.0 0.0 0.2483501215699896 0.0 32 0.0 0.0 0.0 0.29437304619659604 0.0 33 0.0 0.0 0.0 0.35863146926015976 0.0 34 0.0 0.0 0.0 0.4402570336922543 0.0 35 0.0 0.0 0.0 0.5201458839874956 0.0 36 0.0 0.0 0.0 0.7111844390413338 0.0 37 0.0 0.0 0.0 0.9586662035428969 0.0 38 0.0 0.0 0.0 1.2261201806182702 0.0 39 0.0 0.0 0.0 1.6394581451893018 0.0 40 0.0 0.0 0.0 2.1839180270927407 0.0 41 0.0 0.0 0.0 2.598992705800625 0.0 42 0.0 0.0 0.0 3.1156651615144146 0.0 43 0.0 0.0 0.0 3.5958666203542897 0.0 44 0.0 0.0 0.0 4.1229593608891975 0.0 45 0.0 0.0 0.0 4.597082320250087 0.0 46 0.0 0.0 0.0 5.01736714136853 0.0 47 0.0 0.0 0.0 5.448072247308093 0.0 48 0.0 0.0 0.0 5.836227856894755 0.0 49 0.0 0.0 0.0 6.20354289683918 0.0 50 0.0 0.0 0.0 6.5474122959360885 0.0 51 0.0 0.0 0.0 6.884334838485585 0.0 52 0.0 0.0 0.0 7.216915595692949 0.0 53 0.0 0.0 0.0 7.551233067037166 0.0 54 0.0 0.0 0.0 7.876866967697117 0.0 55 0.0 0.0 0.0 8.182528655783258 0.0 56 0.0 0.0 0.0 8.494268843348385 0.0 57 0.0 0.0 0.0 8.809482459187217 0.0 58 0.0 0.0 0.0 9.038728725251824 0.0 59 0.0 0.0 0.0 9.276658562000694 0.0 60 0.0 0.0 0.0 9.535428968391802 0.0 61 0.0 0.0 0.0 9.745571378951025 0.0 62 0.0 0.0 0.0 9.951372004168114 0.0 63 0.0 0.0 0.0 10.201458839874956 0.0 64 0.0 0.0 0.0 10.483674887113581 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position ACCATTT 15 0.00221981 70.0 65 CCGGCGA 15 0.00221981 70.0 21 ACCGCGG 15 0.00221981 70.0 30 GTCTAAC 15 0.00221981 70.0 1 GGCTTTC 15 0.00221981 70.0 13 GGGCGAG 20 7.8984056E-5 70.0 22 ATCCATT 15 0.00221981 70.0 43 TCGGTGA 15 0.00221981 70.0 60 GTACGGT 15 0.00221981 70.0 11 CGTGTGG 25 2.3819819E-4 55.999996 9 TCTGGCG 50 9.695214E-10 55.999996 11 TGCCGGC 45 2.6751877E-8 54.444447 19 CTTGTGC 20 0.0069362405 52.5 1 TCCATTG 20 0.0069362405 52.5 44 TCCATGA 20 0.0069362405 52.5 34 CACGGCG 20 0.0069362405 52.5 7 GTTTCAA 20 0.0069362405 52.5 12 CTTTTAA 20 0.0069362405 52.5 24 AACTTTT 20 0.0069362405 52.5 22 AGCCAAC 20 0.0069362405 52.5 18 >>END_MODULE