FastQCFastQC Report
Wed 25 May 2016
SRR1781013_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1781013_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences115160
Sequences flagged as poor quality0
Sequence length76
%GC49

[WARN]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CATATACACATCTCCGAGCCCACGAGACGAGCACGTATCTCGTATGCCGT3798332.98280653004515No Hit
CTTATACACATCTCCGAGCCCACGAGACGAGCACGTATCTCGTATGCCGT59905.201458839874957No Hit
TCTTATACACATCTCCGAGCCCACGAGACGAGCACGTATCTCGTATGCCG27542.391455366446683No Hit
CACTTATACACATCTCCGAGCCCACGAGACGAGCACGTATCTCGTATGCC25322.1986800972559917No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG25092.178707884682181No Hit
CAAATACACATCTCCGAGCCCACGAGACGAGCACGTATCTCGTATGCCGT23962.080583535949983No Hit
CTCTTATACACATCTCCGAGCCCACGAGACGAGCACGTATCTCGTATGCC8010.6955540118096561No Hit
AAACACATCTCCGAGCCCACGAGACGAGCACGTATCTCGTATGCCGTCTT6260.5435915248350122RNA PCR Primer, Index 2 (95% over 21bp)
ACTTATACACATCTCCGAGCCCACGAGACGAGCACGTATCTCGTATGCCG6070.527092740534908No Hit
TCTTTATACACATCTCCGAGCCCACGAGACGAGCACGTATCTCGTATGCC5020.4359152483501215No Hit
CAATACACATCTCCGAGCCCACGAGACGAGCACGTATCTCGTATGCCGTC4180.36297325460229246No Hit
CATATACAAATCTCCGAGCCCACGAGACGAGCACGTATCTCGTATGCCGT3410.2961097603334491No Hit
CATAAACACATCTCCGAGCCCACGAGACGAGCACGTATCTCGTATGCCGT3220.2796109760333449No Hit
CATATACACATCTCCGAGCCCACGAGACGAGCACGTATCTCGTATGACGT3100.2691906912122265No Hit
ATACACATCTCCGAGCCCACGAGACGAGCACGTATCTCGTATGCCGTCTT2800.24313997915943034TruSeq Adapter, Index 2 (95% over 21bp)
CATTACACATCTCCGAGCCCACGAGACGAGCACGTATCTCGTATGCCGTC2480.21535255296978115No Hit
TCATATACACATCTCCGAGCCCACGAGACGAGCACGTATCTCGTATGCCG2440.211879124696075No Hit
CTAATACACATCTCCGAGCCCACGAGACGAGCACGTATCTCGTATGCCGT2360.20493226814866272No Hit
CATTATACACATCTCCGAGCCCACGAGACGAGCACGTATCTCGTATGCCG2350.2040639110802362No Hit
CATATACACATCTCCGAGCCCACGAGACGCGCACGTATCTCGTATGCCGT2340.20319555401180966No Hit
CATATACACATATCCGAGCCCACGAGACGAGCACGTATCTCGTATGCCGT1890.1641194859326155No Hit
CATATACACATCTCCGAGCCCACGAGACGAGCACGTATCTCGTATGCAGT1870.1623827717957624No Hit
CATATACACATCTCCGAGCCAACGAGACGAGCACGTATCTCGTATGCCGT1760.1528308440430705No Hit
CATATACACATCTCCGAGCCCACGAGACGAGAACGTATCTCGTATGCCGT1750.15196248697464398No Hit
CAACACATCTCCGAGCCCACGAGACGAGCACGTATCTCGTATGCCGTCTT1730.1502257728377909TruSeq Adapter, Index 2 (95% over 21bp)
CATAATACACATCTCCGAGCCCACGAGACGAGCACGTATCTCGTATGCCG1640.1424105592219521No Hit
CATATACACATCTCCGAGCCCACGAGACGAGCACGAATCTCGTATGCCGT1520.13199027440083363No Hit
CAAAAACACATCTCCGAGCCCACGAGACGAGCACGTATCTCGTATGCCGT1490.129385203195554No Hit
CCTTATACACATCTCCGAGCCCACGAGACGAGCACGTATCTCGTATGCCG1260.10941299062174367No Hit
CATATACACATCTCCGAGACCACGAGACGAGCACGTATCTCGTATGCCGT1240.10767627648489057No Hit
CATATACACATCTCCGAGCCCACGAGACGAGCAAGTATCTCGTATGCCGT1240.10767627648489057No Hit
CTATACACATCTCCGAGCCCACGAGACGAGCACGTATCTCGTATGCCGTC1190.1033344911427579No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCCGTAT455.456968E-1270.0000146
AAGTATC650.070.033
AGCCGTC500.070.045
GTATTCA150.0022198170.049
CGTGATC150.0022198170.030
ATCAGCT150.0022198170.054
GTGATCA150.0022198170.031
CATTACA207.8984056E-570.01
GCTCTTC150.0022198170.051
ACGATCA301.01612386E-770.027
TCTACAG150.0022198170.050
CTTCTAT150.0022198170.054
CTCGAAA207.8984056E-570.039
CAAGAGA301.01612386E-770.021
GCGTGAT150.0022198170.029
AGTAGCG150.0022198170.070
GTAAGAC150.0022198170.042
TCCTTTG150.0022198170.031
TACAGCT150.0022198170.052
CGATCAC353.6743586E-970.028
Produced by FastQC (version 0.11.5)