##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1781013_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 115160 Sequences flagged as poor quality 0 Sequence length 76 %GC 49 >>END_MODULE >>Per base sequence quality warn #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 28.344190691212226 32.0 27.0 32.0 13.0 32.0 2 21.29320076415422 22.0 13.0 32.0 8.0 32.0 3 28.465708579367835 32.0 27.0 32.0 13.0 32.0 4 30.838911080236194 32.0 32.0 32.0 27.0 32.0 5 30.142827370614796 32.0 32.0 32.0 27.0 32.0 6 33.95962139631816 37.0 32.0 37.0 27.0 37.0 7 33.926701979854116 37.0 37.0 37.0 27.0 37.0 8 32.02197811740188 37.0 32.0 37.0 13.0 37.0 9 32.347004168113926 37.0 32.0 37.0 13.0 37.0 10-11 34.483857242097955 37.0 37.0 37.0 29.5 37.0 12-13 34.19621396318166 37.0 37.0 37.0 27.0 37.0 14-15 34.07539510246613 37.0 37.0 37.0 27.0 37.0 16-17 33.19098211184439 37.0 34.5 37.0 22.0 37.0 18-19 33.81740187565127 37.0 34.5 37.0 24.5 37.0 20-21 34.190161514414726 37.0 37.0 37.0 27.0 37.0 22-23 34.004862799583194 37.0 34.5 37.0 24.5 37.0 24-25 34.034760333449114 37.0 34.5 37.0 27.0 37.0 26-27 34.4524010072942 37.0 37.0 37.0 29.5 37.0 28-29 34.37942427926363 37.0 37.0 37.0 27.0 37.0 30-31 33.624418200764154 37.0 34.5 37.0 24.5 37.0 32-33 33.28450850989927 37.0 34.5 37.0 20.0 37.0 34-35 33.773953629732546 37.0 37.0 37.0 24.5 37.0 36-37 33.74492879472039 37.0 37.0 37.0 24.5 37.0 38-39 33.36148836401529 37.0 34.5 37.0 22.0 37.0 40-41 33.74139023966655 37.0 34.5 37.0 24.5 37.0 42-43 33.89334404307051 37.0 37.0 37.0 24.5 37.0 44-45 34.130922195206665 37.0 37.0 37.0 27.0 37.0 46-47 33.42128777353248 37.0 34.5 37.0 22.0 37.0 48-49 32.44101684612713 37.0 32.0 37.0 17.5 37.0 50-51 33.369620527961104 37.0 37.0 37.0 22.0 37.0 52-53 32.972095345606114 37.0 34.5 37.0 17.5 37.0 54-55 32.96634682181313 37.0 32.0 37.0 22.0 37.0 56-57 32.75747655435916 37.0 32.0 37.0 17.5 37.0 58-59 32.796800104202845 37.0 32.0 37.0 17.5 37.0 60-61 33.07332841264328 37.0 32.0 37.0 17.5 37.0 62-63 33.867440951719345 37.0 37.0 37.0 27.0 37.0 64-65 33.99424279263633 37.0 37.0 37.0 27.0 37.0 66-67 33.735698159083015 37.0 37.0 37.0 22.0 37.0 68-69 32.91872177839528 37.0 32.0 37.0 17.5 37.0 70-71 30.27588138242445 37.0 24.5 37.0 13.0 37.0 72-73 32.07577717957624 37.0 32.0 37.0 13.0 37.0 74-75 33.00837964571032 37.0 34.5 37.0 13.0 37.0 76 33.48897186523098 37.0 37.0 37.0 22.0 37.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 11101 1 -0.15513996328802193 11101 2 0.009969482709689004 11101 3 0.13505057496257322 11101 4 0.040020826393572406 11101 5 -1.0565509495421068 11101 6 -0.7209312731045046 11101 7 0.5495312502887231 11101 8 -1.775138124625233 11101 9 0.33530710227869776 11101 10-11 -0.42734985327494 11101 12-13 0.7517236833473646 11101 14-15 -0.10069423836456082 11101 16-17 -0.26585236338226537 11101 18-19 -0.5790414159286073 11101 20-21 -0.07931083142700146 11101 22-23 0.16229666230683648 11101 24-25 -0.09000002001457119 11101 26-27 -0.08099862662119506 11101 28-29 0.7836148615540921 11101 30-31 -1.1962593498293899 11101 32-33 0.5173370208359813 11101 34-35 0.4932421473824036 11101 36-37 -0.6389424400959598 11101 38-39 -0.9323074989400766 11101 40-41 -0.08758639377097666 11101 42-43 0.4601721646417971 11101 44-45 0.34543747428604377 11101 46-47 0.07971149322406745 11101 48-49 0.2425236083336344 11101 50-51 -0.47052489538265263 11101 52-53 -0.6162902655119069 11101 54-55 -1.174539332906832 11101 56-57 -0.659422590999764 11101 58-59 0.8274633419934005 11101 60-61 -1.0884451348450916 11101 62-63 -1.0697520309238087 11101 64-65 0.20824253050277264 11101 66-67 -0.47354552133447925 11101 68-69 -1.0964491837508064 11101 70-71 -1.828079917305633 11101 72-73 1.0335222341576795 11101 74-75 0.8572083172062648 11101 76 0.7626879299109603 11102 1 -0.18554350115424612 11102 2 -0.29480295141598134 11102 3 0.25721751802504045 11102 4 -0.4578601391490871 11102 5 -0.7180582279648071 11102 6 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11103 46-47 -0.26491369543935406 11103 48-49 1.3085977320968425 11103 50-51 1.0703941051204566 11103 52-53 1.1324100531196493 11103 54-55 1.4539194965414381 11103 56-57 1.1270104125219547 11103 58-59 0.5787630233618373 11103 60-61 1.315965396766515 11103 62-63 -0.05566516189814763 11103 64-65 0.14896029001979372 11103 66-67 0.4656629307869409 11103 68-69 0.32062275990171685 11103 70-71 -1.627761285744338 11103 72-73 1.640683059245724 11103 74-75 1.2665660230590206 11103 76 0.7033218390171143 11104 1 -0.8892472048579485 11104 2 1.5693945794482183 11104 3 0.3930199871608444 11104 4 0.02362134233239388 11104 5 0.10910226586235439 11104 6 -0.04357730221819622 11104 7 0.8651752524998884 11104 8 -0.5858991343912159 11104 9 -0.5950411564277722 11104 10-11 -0.015059453422360036 11104 12-13 -0.47482873972247575 11104 14-15 0.6133282418270696 11104 16-17 0.47719167568166654 11104 18-19 0.08108388350017748 11104 20-21 0.07777411421515268 11104 22-23 -0.01809766236608823 11104 24-25 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0.6122281419944429 11105 68-69 -0.09389864530380265 11105 70-71 1.281782164689453 11105 72-73 1.1177844672957846 11105 74-75 0.30553629755767275 11105 76 0.9045734390740066 11106 1 -0.029887845498588916 11106 2 1.76208727214091 11106 3 -1.5210607602532384 11106 4 0.3756400276844154 11106 5 0.25708358051033287 11106 6 -0.20156862687618826 11106 7 1.039849927174565 11106 8 -1.110757325916076 11106 9 -0.47792403931065763 11106 10-11 -0.015893620923186802 11106 12-13 0.12435377612671061 11106 14-15 -0.5409927458272463 11106 16-17 -0.2544566392999812 11106 18-19 -0.025189056106093233 11106 20-21 0.5505802536879614 11106 22-23 -0.16616239376415365 11106 24-25 -0.006095219691871989 11106 26-27 0.471845262043594 11106 28-29 -0.28102813965607965 11106 30-31 -0.8040649165901783 11106 32-33 -0.08098891704219113 11106 34-35 0.24979720990761223 11106 36-37 -1.7078210201685664 11106 38-39 -0.132589870864237 11106 40-41 -0.5227416983292699 11106 42-43 0.0024271061504705926 11106 44-45 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12-13 -0.029823128050189496 21610 14-15 0.7620375572030511 21610 16-17 1.0072141223707831 21610 18-19 0.3333285524115084 21610 20-21 -0.5395098364021251 21610 22-23 -0.31972504732681273 21610 24-25 0.05348708989043871 21610 26-27 0.32503936523769283 21610 28-29 0.5277351691072383 21610 30-31 -0.22011733264258737 21610 32-33 0.28633295027967876 21610 34-35 1.3677333278437374 21610 36-37 1.1967653844178372 21610 38-39 1.134404396130023 21610 40-41 0.1271354515478862 21610 42-43 0.754269857993215 21610 44-45 0.7311226845981196 21610 46-47 0.07948535120688405 21610 48-49 1.7295204423453896 21610 50-51 -0.5095343933473657 21610 52-53 1.7913307204672861 21610 54-55 -0.7078952804418819 21610 56-57 1.0372078929441528 21610 58-59 1.2683272044990588 21610 60-61 0.5703065612200433 21610 62-63 0.7978418180133247 21610 64-65 0.11235831521557316 21610 66-67 0.636585641035488 21610 68-69 -0.007688442004990748 21610 70-71 1.1641181831014222 21610 72-73 0.21703648110476337 21610 74-75 -0.40342035760151873 21610 76 1.1740402591737364 21611 1 -0.38082969644043985 21611 2 -1.2252182151645776 21611 3 0.9003151611226841 21611 4 0.3260904781348657 21611 5 -0.208518385091633 21611 6 -0.8539027792103369 21611 7 -0.2779223905977517 21611 8 1.772125556966806 21611 9 0.4958679344813177 21611 10-11 0.36821776318819843 21611 12-13 -0.09042918865625182 21611 14-15 -0.19250789734239504 21611 16-17 0.31024442540108765 21611 18-19 0.17423764332060188 21611 20-21 -0.3955704424627342 21611 22-23 -0.1682098958116569 21611 24-25 -0.12833109192773406 21611 26-27 -0.394657604459276 21611 28-29 0.9216745630466292 21611 30-31 0.18897357644831914 21611 32-33 0.6120905260372567 21611 34-35 -0.011054550944145092 21611 36-37 -0.06838613073367839 21611 38-39 -0.8049895432639147 21611 40-41 0.49834757276000374 21611 42-43 -1.1093665056431448 21611 44-45 -0.32190761843218496 21611 46-47 -0.26142373970220945 21611 48-49 -0.07350986068490784 21611 50-51 -1.358019241832217 21611 52-53 -0.7920026128660496 21611 54-55 -0.010925583472179312 21611 56-57 -0.21279210705584717 21611 58-59 -0.18621825004639447 21611 60-61 0.6460641369776141 21611 62-63 0.6160236361951448 21611 64-65 0.150237103094355 21611 66-67 -0.0452325407826919 21611 68-69 -0.42435510867165505 21611 70-71 -0.1843666653834255 21611 72-73 -0.12387260980433012 21611 74-75 -0.7216021757833389 21611 76 -0.8714142862808103 21612 1 -3.1272646340518477 21612 2 -0.9676424575888198 21612 3 2.054058476630704 21612 4 0.012364987938784822 21612 5 1.385064502608902 21612 6 -0.7683412818841333 21612 7 0.8526480193844179 21612 8 2.7819294785354387 21612 9 -0.11464899956502705 21612 10-11 0.3298933425108359 21612 12-13 -1.3500102938255978 21612 14-15 0.6795954894668697 21612 16-17 1.0499948710338813 21612 18-19 0.49130537950598807 21612 20-21 0.6688234969312035 21612 22-23 0.4536706745983281 21612 24-25 1.182497785077608 21612 26-27 0.47343508716282656 21612 28-29 0.7010863277525132 21612 30-31 1.2533675158422568 21612 32-33 0.2636056775524054 21612 34-35 0.07829482516994091 21612 36-37 0.19698820081712398 21612 38-39 0.31176624996246716 21612 40-41 0.7775365210665939 21612 42-43 1.1526655799183558 21612 44-45 0.8093312407478521 21612 46-47 0.7564015722407547 21612 48-49 0.18161491649868822 21612 50-51 1.170278440877226 21612 52-53 0.4807246598612238 21612 54-55 0.6004826161713126 21612 56-57 1.0909066451723248 21612 58-59 0.12750724014967574 21612 60-61 0.26482527779757703 21612 62-63 0.3969951156960434 21612 64-65 0.21307132769328518 21612 66-67 1.0298565857770186 21612 68-69 1.1629889198488428 21612 70-71 2.8486101617110506 21612 72-73 0.9951113474149196 21612 74-75 1.0072302662844024 21612 76 0.2310812573912031 >>END_MODULE >>Per sequence quality scores pass #Quality Count 12 1.0 13 0.0 14 0.0 15 4.0 16 6.0 17 11.0 18 22.0 19 67.0 20 149.0 21 321.0 22 632.0 23 996.0 24 1403.0 25 1815.0 26 2191.0 27 2721.0 28 3214.0 29 3983.0 30 5121.0 31 6779.0 32 9659.0 33 15437.0 34 24279.0 35 28771.0 36 7578.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 13.21696843385003 11.83101037731753 7.609743389344796 67.34227779948765 2 7.97412295936089 64.27318513372698 10.996873914553666 16.75581799235846 3 8.795588746092394 19.359152483501216 58.06356373740882 13.781695032997568 4 6.94598819034387 58.60020840569642 22.933310177144843 11.520493226814867 5 6.786210489753386 22.253386592566862 65.07294199374782 5.88746092393192 6 12.175234456408475 68.87287252518236 11.779263633205975 7.172629385203195 7 11.261918407752827 18.795915177408432 12.804147345386339 57.138019069452405 8 10.586232947489991 68.09628426783839 8.626334025130472 12.691148759541157 9 10.248439114615444 14.796932936201252 7.753627593153814 67.2010003560295 10-11 10.728163981260773 39.95111128478328 35.8910901836149 13.42963455034105 12-13 11.14102118791247 12.263372698853768 39.42037165682529 37.175234456408475 14-15 11.310399923584246 10.550150009334878 12.7883500709885 65.35109999609237 16-17 38.96405001736714 36.070250086835706 8.447377561653353 16.5183223341438 18-19 40.67029064163461 15.579763631153448 8.570324507854359 35.179621219357585 20-21 13.034907954150746 11.155349079541507 8.667071899965267 67.14267106634249 22-23 11.551754081278222 38.60672108370962 8.640587009378256 41.2009378256339 24-25 39.14987843001042 38.58414379993053 8.57198680097256 13.693990969086489 26-27 41.40673845085099 41.265196248697464 8.775616533518583 8.552448766932963 28-29 39.46934238748165 15.147318055905313 8.971074774876476 36.41226478173655 30-31 41.28453512455006 37.849584688396895 8.82162659731057 12.044253589742477 32-33 15.634334838485586 38.54723862452241 9.54975686002084 36.26866967697117 34-35 36.27257728377909 15.455453282389719 10.391194859326156 37.88077457450504 36-37 15.067297672803056 36.009899270580064 37.59942688433484 11.323376172282043 38-39 10.715960402917679 14.931833970128515 39.67132684960056 34.68087877735325 40-41 10.450858820055924 10.015370187047361 40.52909915073204 39.004671842164676 42-43 38.3311116803946 9.772523001706404 38.38147879587858 13.514886522020417 44-45 12.142236887808266 37.26163598471692 40.73680097255992 9.8593261549149 46-47 37.98836351005167 11.745039294863444 15.865572489253616 34.401024705831276 48-49 37.393241254141174 9.432110772918122 11.52929325384592 41.645354719094776 50-51 13.746960750260506 9.898836401528309 38.94277526919069 37.4114275790205 52-53 9.283766651035968 10.53726184893798 66.2281387311346 13.950832768891436 54-55 8.989666550885724 10.62695380340396 43.1117575547065 37.27162209100382 56-57 36.972907259465096 9.597950677318513 15.860541854810698 37.5686002084057 58-59 11.507617761606829 11.99390405397778 62.47736814911622 14.021110035299175 60-61 36.87000694685655 37.27249044807225 16.391542202153524 9.465960402917679 62-63 11.105852726641196 70.51189649183745 8.765196248697464 9.617054532823897 64-65 8.917158735672109 72.89944425147621 8.288034039597083 9.895362973254603 66-67 9.1589961792289 73.17514762070164 7.831278221604723 9.834577978464745 68-69 10.472386245224035 71.83397012851684 7.735758944077804 9.957884682181312 70-71 36.502257728377906 45.9491142757902 7.609412990621743 9.939215005210142 72-73 71.06974765105333 11.590597266364473 7.5700342138626935 9.7696208687195 74-75 73.58805140673846 9.45944772490448 7.2763980548801666 9.676102813476902 76 74.19069121222647 8.97707537339354 6.826154914901007 10.006078499478987 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.5 11 2.0 12 3.0 13 3.5 14 6.0 15 5.5 16 7.0 17 11.0 18 16.0 19 24.5 20 34.5 21 41.0 22 48.5 23 69.0 24 83.5 25 85.0 26 125.5 27 197.5 28 265.5 29 302.0 30 359.0 31 457.0 32 609.0 33 720.0 34 751.0 35 902.5 36 1139.5 37 1256.0 38 1414.5 39 1734.5 40 2019.5 41 2329.5 42 2516.0 43 2710.0 44 3173.5 45 3742.5 46 4042.0 47 5237.0 48 10792.5 49 32790.0 50 50427.0 51 27163.5 52 3240.0 53 2212.0 54 1844.0 55 1645.0 56 1268.0 57 1004.0 58 918.0 59 819.5 60 666.0 61 605.0 62 599.0 63 543.0 64 432.0 65 360.5 66 313.0 67 282.0 68 249.5 69 196.5 70 172.0 71 168.0 72 161.5 73 132.5 74 87.0 75 64.0 76 58.0 77 46.0 78 41.5 79 43.0 80 36.5 81 29.0 82 24.5 83 21.0 84 13.5 85 7.5 86 6.5 87 4.0 88 5.0 89 6.5 90 5.0 91 1.5 92 0.0 93 1.5 94 5.0 95 7.0 96 7.0 97 11.0 98 27.0 99 1263.0 100 2487.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.004341785342132685 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.001736714136853074 8 8.68357068426537E-4 9 8.68357068426537E-4 10-11 4.341785342132685E-4 12-13 0.0 14-15 4.341785342132685E-4 16-17 0.0 18-19 8.68357068426537E-4 20-21 0.0 22-23 0.0 24-25 0.0 26-27 0.0 28-29 8.68357068426537E-4 30-31 0.0047759638763459535 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.0 40-41 0.001736714136853074 42-43 0.0047759638763459535 44-45 0.0 46-47 0.004341785342132685 48-49 0.0047759638763459535 50-51 0.0 52-53 0.001736714136853074 54-55 0.0 56-57 0.0 58-59 0.0013025356026398055 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 0.0 70-71 0.0 72-73 0.001736714136853074 74-75 0.0 76 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 76 115160.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 26.820944772490446 #Duplication Level Percentage of deduplicated Percentage of total 1 74.16712532780781 19.89232372351511 2 11.17298539838767 5.993400486279958 3 5.996050118172694 4.824591872177839 4 3.3347362968239063 3.5776311219173325 5 1.8713374558875902 2.5095519277526916 6 1.1072619548677438 1.781868704411254 7 0.712273772137145 1.337269885376867 8 0.39498818273059866 0.8475164987843 9 0.24605821219283192 0.5939562348037513 >10 0.815877229902548 3.7721431052448766 >50 0.0712273772137145 1.3520319555401181 >100 0.07770259332405219 4.281868704411254 >500 0.012950432220675365 2.202153525529698 >1k 0.012950432220675365 8.849426884334838 >5k 0.003237608055168841 5.201458839874957 >10k+ 0.003237608055168841 32.98280653004515 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source CATATACACATCTCCGAGCCCACGAGACGAGCACGTATCTCGTATGCCGT 37983 32.98280653004515 No Hit CTTATACACATCTCCGAGCCCACGAGACGAGCACGTATCTCGTATGCCGT 5990 5.201458839874957 No Hit TCTTATACACATCTCCGAGCCCACGAGACGAGCACGTATCTCGTATGCCG 2754 2.391455366446683 No Hit CACTTATACACATCTCCGAGCCCACGAGACGAGCACGTATCTCGTATGCC 2532 2.1986800972559917 No Hit GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG 2509 2.178707884682181 No Hit CAAATACACATCTCCGAGCCCACGAGACGAGCACGTATCTCGTATGCCGT 2396 2.080583535949983 No Hit CTCTTATACACATCTCCGAGCCCACGAGACGAGCACGTATCTCGTATGCC 801 0.6955540118096561 No Hit AAACACATCTCCGAGCCCACGAGACGAGCACGTATCTCGTATGCCGTCTT 626 0.5435915248350122 RNA PCR Primer, Index 2 (95% over 21bp) ACTTATACACATCTCCGAGCCCACGAGACGAGCACGTATCTCGTATGCCG 607 0.527092740534908 No Hit TCTTTATACACATCTCCGAGCCCACGAGACGAGCACGTATCTCGTATGCC 502 0.4359152483501215 No Hit CAATACACATCTCCGAGCCCACGAGACGAGCACGTATCTCGTATGCCGTC 418 0.36297325460229246 No Hit CATATACAAATCTCCGAGCCCACGAGACGAGCACGTATCTCGTATGCCGT 341 0.2961097603334491 No Hit CATAAACACATCTCCGAGCCCACGAGACGAGCACGTATCTCGTATGCCGT 322 0.2796109760333449 No Hit CATATACACATCTCCGAGCCCACGAGACGAGCACGTATCTCGTATGACGT 310 0.2691906912122265 No Hit ATACACATCTCCGAGCCCACGAGACGAGCACGTATCTCGTATGCCGTCTT 280 0.24313997915943034 TruSeq Adapter, Index 2 (95% over 21bp) CATTACACATCTCCGAGCCCACGAGACGAGCACGTATCTCGTATGCCGTC 248 0.21535255296978115 No Hit TCATATACACATCTCCGAGCCCACGAGACGAGCACGTATCTCGTATGCCG 244 0.211879124696075 No Hit CTAATACACATCTCCGAGCCCACGAGACGAGCACGTATCTCGTATGCCGT 236 0.20493226814866272 No Hit CATTATACACATCTCCGAGCCCACGAGACGAGCACGTATCTCGTATGCCG 235 0.2040639110802362 No Hit CATATACACATCTCCGAGCCCACGAGACGCGCACGTATCTCGTATGCCGT 234 0.20319555401180966 No Hit CATATACACATATCCGAGCCCACGAGACGAGCACGTATCTCGTATGCCGT 189 0.1641194859326155 No Hit CATATACACATCTCCGAGCCCACGAGACGAGCACGTATCTCGTATGCAGT 187 0.1623827717957624 No Hit CATATACACATCTCCGAGCCAACGAGACGAGCACGTATCTCGTATGCCGT 176 0.1528308440430705 No Hit CATATACACATCTCCGAGCCCACGAGACGAGAACGTATCTCGTATGCCGT 175 0.15196248697464398 No Hit CAACACATCTCCGAGCCCACGAGACGAGCACGTATCTCGTATGCCGTCTT 173 0.1502257728377909 TruSeq Adapter, Index 2 (95% over 21bp) CATAATACACATCTCCGAGCCCACGAGACGAGCACGTATCTCGTATGCCG 164 0.1424105592219521 No Hit CATATACACATCTCCGAGCCCACGAGACGAGCACGAATCTCGTATGCCGT 152 0.13199027440083363 No Hit CAAAAACACATCTCCGAGCCCACGAGACGAGCACGTATCTCGTATGCCGT 149 0.129385203195554 No Hit CCTTATACACATCTCCGAGCCCACGAGACGAGCACGTATCTCGTATGCCG 126 0.10941299062174367 No Hit CATATACACATCTCCGAGACCACGAGACGAGCACGTATCTCGTATGCCGT 124 0.10767627648489057 No Hit CATATACACATCTCCGAGCCCACGAGACGAGCAAGTATCTCGTATGCCGT 124 0.10767627648489057 No Hit CTATACACATCTCCGAGCCCACGAGACGAGCACGTATCTCGTATGCCGTC 119 0.1033344911427579 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.010420284821118444 0.0 2 0.0 0.0 0.0 0.013893713094824591 0.0 3 0.0 0.0 0.0 0.014762070163251128 0.0 4 0.0 0.0 0.0 0.019103855505383814 0.0 5 0.0 0.0 0.0 0.027787426189649182 0.0 6 0.0 0.0 0.0 0.027787426189649182 0.0 7 0.0 0.0 0.0 0.027787426189649182 0.0 8 0.0 0.0 0.0 0.027787426189649182 0.0 9 0.0 0.0 0.0 0.03473428273706148 0.0 10 0.0 0.0 0.0 0.03473428273706148 0.0 11 0.0 0.0 0.0 0.035602639805488015 0.0 12 0.0 0.0 0.0 0.035602639805488015 0.0 13 0.0 0.0 0.0 0.03647099687391455 0.0 14 0.0 0.0 0.0 0.0399444251476207 0.0 15 0.0 0.0 0.0 0.04168113928447378 0.0 16 0.0 0.0 0.0 0.04168113928447378 0.0 17 0.0 0.0 0.0 0.04254949635290031 0.0 18 0.0 0.0 0.0 0.05210142410559222 0.0 19 0.0 0.0 0.0 0.05644320944772491 0.0 20 0.0 0.0 0.0 0.06078499478985759 0.0 21 0.0 0.0 0.0 0.061653351858284126 0.0 22 0.0 0.0 0.0 0.06512678013199028 0.0 23 0.0 0.0 0.0 0.07467870788468219 0.0 24 0.0 0.0 0.0 0.07641542202153526 0.0 25 0.0 0.0 0.0 0.08944077804793331 0.0 26 0.0 0.0 0.0 0.09030913511635985 0.0 27 0.0 0.0 0.0 0.10159777700590483 0.0 28 0.0 0.0 0.0 0.12330670371656825 0.0 29 0.0 0.0 0.0 0.14675234456408476 0.0 30 0.0 0.0 0.0 0.17193469954845433 0.0 31 0.0 0.0 0.0 0.2066689822855158 0.0 32 0.0 0.0 0.0 0.25703369225425493 0.0 33 0.0 0.0 0.0 0.3247655435915248 0.0 34 0.0 0.0 0.0 0.40986453629732544 0.0 35 0.0 0.0 0.0 0.4967002431399792 0.0 36 0.0 0.0 0.0 0.6998957971517888 0.0 37 0.0 0.0 0.0 0.9968739145536645 0.0 38 0.0 0.0 0.0 1.2869051754081278 0.0 39 0.0 0.0 0.0 1.714136853073984 0.0 40 0.0 0.0 0.0 2.2629385203195556 0.0 41 0.0 0.0 0.0 2.697117054532824 0.0 42 0.0 0.0 0.0 3.2268148662730116 0.0 43 0.0 0.0 0.0 3.72872525182355 0.0 44 0.0 0.0 0.0 4.266238277179577 0.0 45 0.0 0.0 0.0 4.788120875303925 0.0 46 0.0 0.0 0.0 5.2335880514067386 0.0 47 0.0 0.0 0.0 5.6816602987148315 0.0 48 0.0 0.0 0.0 6.101076762764849 0.0 49 0.0 0.0 0.0 6.495310871830497 0.0 50 0.0 0.0 0.0 6.854810698159083 0.0 51 0.0 0.0 0.0 7.217783952761375 0.0 52 0.0 0.0 0.0 7.572941993747829 0.0 53 0.0 0.0 0.0 7.940257033692254 0.0 54 0.0 0.0 0.0 8.280653004515457 0.0 55 0.0 0.0 0.0 8.588051406738451 0.0 56 0.0 0.0 0.0 8.900659951372004 0.0 57 0.0 0.0 0.0 9.221952066689823 0.0 58 0.0 0.0 0.0 9.456408475164988 0.0 59 0.0 0.0 0.0 9.705626953803405 0.0 60 0.0 0.0 0.0 9.97481764501563 0.0 61 0.0 0.0 0.0 10.178881556095867 0.0 62 0.0 0.0 0.0 10.390760680791942 0.0 63 0.0 0.0 0.0 10.62695380340396 0.0 64 0.0 0.0 0.0 10.909169850642584 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GCCGTAT 45 5.456968E-12 70.00001 46 AAGTATC 65 0.0 70.0 33 AGCCGTC 50 0.0 70.0 45 GTATTCA 15 0.00221981 70.0 49 CGTGATC 15 0.00221981 70.0 30 ATCAGCT 15 0.00221981 70.0 54 GTGATCA 15 0.00221981 70.0 31 CATTACA 20 7.8984056E-5 70.0 1 GCTCTTC 15 0.00221981 70.0 51 ACGATCA 30 1.01612386E-7 70.0 27 TCTACAG 15 0.00221981 70.0 50 CTTCTAT 15 0.00221981 70.0 54 CTCGAAA 20 7.8984056E-5 70.0 39 CAAGAGA 30 1.01612386E-7 70.0 21 GCGTGAT 15 0.00221981 70.0 29 AGTAGCG 15 0.00221981 70.0 70 GTAAGAC 15 0.00221981 70.0 42 TCCTTTG 15 0.00221981 70.0 31 TACAGCT 15 0.00221981 70.0 52 CGATCAC 35 3.6743586E-9 70.0 28 >>END_MODULE