FastQCFastQC Report
Wed 25 May 2016
SRR1781012_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1781012_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences287417
Sequences flagged as poor quality0
Sequence length76
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG3550512.353131512749767No Hit
CTTATACACATCTGACGCTGCCGACGAACATAAGGGTGTAGATCTCGGTG6540.22754395181913387No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTCCGT150.002223640469.996521
CGAGAAC200.00693856552.51566734
CCGAACT200.006943343752.50652726
GAACCTT352.0819998E-550.00621854
GTACATG305.873444E-446.6643451
TACATGC350.001254504439.9980132
GATCCCC350.001255578539.9910556
TGGTCGC1051.2732926E-1136.66484549
CGGTGGT1151.8189894E-1236.51357346
CGTTGAA400.002414951335.00435342
AGATCTC1201.8189894E-1235.0043540
GCTGCAC501.707798E-434.9982669
GCGTCAA400.002419078734.99217259
CGTCAAA400.002419078734.99217260
TCGGTGG1300.034.99217245
AATAGCG501.709546E-434.9921762
GTCGCCG1154.0017767E-1133.4824251
TCTCGGT1500.032.66504343
CTCGGTG1400.032.49838644
TCTAGTT552.9826837E-431.82767533
Produced by FastQC (version 0.11.5)