FastQCFastQC Report
Wed 25 May 2016
SRR1781012_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1781012_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences287417
Sequences flagged as poor quality0
Sequence length76
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CATATACACATCTCCGAGCCCACGAGACAATGGTAGATCTCGTATGCCGT195006.784567370753991No Hit
CTTATACACATCTCCGAGCCCACGAGACAATGGTAGATCTCGTATGCCGT31931.110929416144487No Hit
TCTTATACACATCTCCGAGCCCACGAGACAATGGTAGATCTCGTATGCCG15090.5250211365368088No Hit
CAAATACACATCTCCGAGCCCACGAGACAATGGTAGATCTCGTATGCCGT13990.48674921803511967No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG11500.40011551160856873No Hit
CACTTATACACATCTCCGAGCCCACGAGACAATGGTAGATCTCGTATGCC11350.39489661363106565No Hit
CTCTTATACACATCTCCGAGCCCACGAGACAATGGTAGATCTCGTATGCC4070.14160609845624997No Hit
AAACACATCTCCGAGCCCACGAGACAATGGTAGATCTCGTATGCCGTCTT3580.12455769839640662RNA PCR Primer, Index 47 (95% over 21bp)
ACTTATACACATCTCCGAGCCCACGAGACAATGGTAGATCTCGTATGCCG2960.10298625342272726No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AACTCGT150.002222435370.0060937
AAAACGG207.92193E-569.9939167
GCACGTG150.002223969769.9939049
TGCCTTA150.002223969769.99390411
ATATACA25250.063.0638242
CATATAC25400.062.553611
AGGGGGG32300.061.97603670
CCGACTT401.0626536E-861.2553347
TGCCGAC401.0626536E-861.2553345
ACGTATG700.060.00522240
CGACTTC553.6379788E-1157.2777148
GGATCCA252.3869841E-456.0048745
CACGTAT700.055.00478739
CGTATGA1150.054.78737641
CAGCTTG452.6922862E-854.4491854
CGTCTTC37050.054.2287448
GCCGTCT36700.054.1736546
CCGTCTT36450.054.1610947
TATGCCG37300.053.95911843
TGCCGTC36950.053.9018545