FastQCFastQC Report
Wed 25 May 2016
SRR1781011_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1781011_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences94496
Sequences flagged as poor quality0
Sequence length76
%GC83

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG5940262.86192008127328No Hit
CTTATACACATCTGACGCTGCCGACGAACATAAGGGTGTAGATCTCGGTG12621.335506264815442No Hit
TCTTATACACATCTGACGCTGCCGACGAACATAAGGGTGTAGATCTCGGT1240.13122248560785643No Hit
GGTGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG1200.12698950220115138No Hit
ATACACATCTGACGCTGCCGACGAACATAAGGGTGTAGATCTCGGTGGTC1100.11640704368438876Illumina Single End PCR Primer 1 (95% over 22bp)
GTGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG980.10370809346427362No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCCCCCG150.002212378770.04928632
TAGAACA150.002217035770.01218439
TGGCGGC150.002217035770.01218419
AATGGAT150.002217035770.01218465
GCGCGTG150.002221769.975126
CTATATG150.002221769.9751261
TATGCGC150.002221769.975123
CGCGTCG150.002221769.975127
GAGATGC150.002221769.9751255
AGATGCT150.002221769.9751256
CTTGTGC252.3839631E-455.98011
TGCGCGT452.6757334E-854.4250955
ATAGGGG200.006913106452.53696430
TCAGGGG200.006913106452.53696429
CAGGGGG200.006913106452.53696430
CCTCAGG200.006913106452.53696427
GGGGGAC200.006913106452.53696432
CTCAGGG200.006913106452.53696428
TGCGGGC200.006927616452.50913619
CCCACCT200.006927616452.50913623