FastQCFastQC Report
Wed 25 May 2016
SRR1781011_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1781011_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences94496
Sequences flagged as poor quality0
Sequence length76
%GC47

[WARN]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CATATACACATCTCCGAGCCCACGAGACGTTAACAGATCTCGTATGCCGT3413036.1179309177108No Hit
CTTATACACATCTCCGAGCCCACGAGACGTTAACAGATCTCGTATGCCGT56345.962157128344057No Hit
TCTTATACACATCTCCGAGCCCACGAGACGTTAACAGATCTCGTATGCCG28152.9789620724686756No Hit
CACTTATACACATCTCCGAGCCCACGAGACGTTAACAGATCTCGTATGCC23952.534498814764646No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG20352.153530308161192No Hit
CAAATACACATCTCCGAGCCCACGAGACGTTAACAGATCTCGTATGCCGT20192.1365983745343717No Hit
CTCTTATACACATCTCCGAGCCCACGAGACGTTAACAGATCTCGTATGCC8450.894217744666441No Hit
ACTTATACACATCTCCGAGCCCACGAGACGTTAACAGATCTCGTATGCCG5980.6328310193024043No Hit
AAACACATCTCCGAGCCCACGAGACGTTAACAGATCTCGTATGCCGTCTT4730.5005502878428716Illumina PCR Primer Index 9 (95% over 22bp)
TCTTTATACACATCTCCGAGCCCACGAGACGTTAACAGATCTCGTATGCC4680.4952590585844904No Hit
CAATACACATCTCCGAGCCCACGAGACGTTAACAGATCTCGTATGCCGTC3820.40424991534033183No Hit
CATATACAAATCTCCGAGCCCACGAGACGTTAACAGATCTCGTATGCCGT3020.3195902472062309No Hit
CATATACACATCTCCGAGCCCACGAGACGTTAACAGATCTCGTATGACGT3020.3195902472062309No Hit
CATAAACACATCTCCGAGCCCACGAGACGTTAACAGATCTCGTATGCCGT2910.3079495428377921No Hit
ATACACATCTCCGAGCCCACGAGACGTTAACAGATCTCGTATGCCGTCTT2850.3016000677277345Illumina PCR Primer Index 9 (95% over 22bp)
CTAATACACATCTCCGAGCCCACGAGACGTTAACAGATCTCGTATGCCGT2360.2497460209955977No Hit
TCATATACACATCTCCGAGCCCACGAGACGTTAACAGATCTCGTATGCCG2150.22752285811039621No Hit
CATATACACATCTCCGAGCCCACGAGACGTTAACAGCTCTCGTATGCCGT2140.22646461225871994No Hit
CATTATACACATCTCCGAGCCCACGAGACGTTAACAGATCTCGTATGCCG2060.21799864544530984No Hit
CATTACACATCTCCGAGCCCACGAGACGTTAACAGATCTCGTATGCCGTC1970.20847443278022348No Hit
CATATACACATATCCGAGCCCACGAGACGTTAACAGATCTCGTATGCCGT1730.18307653233999324No Hit
CATATACACATCTCCGAGCCCACGAGACGTTAACAGATATCGTATGCCGT1680.17778530308161192No Hit
CATATACACATCTCCGAGCCCACGAGACGTTAACAGATCTCGTATGCAGT1600.16931933626820184No Hit
CCTTATACACATCTCCGAGCCCACGAGACGTTAACAGATCTCGTATGCCG1470.15556214019641043No Hit
CAACACATCTCCGAGCCCACGAGACGTTAACAGATCTCGTATGCCGTCTT1430.15132915678970538Illumina PCR Primer Index 9 (95% over 22bp)
CATATACACATCTCCGAGCCCACGAGACGATAACAGATCTCGTATGCCGT1400.1481544192346766No Hit
CATATACACATCTCCGAGCCAACGAGACGTTAACAGATCTCGTATGCCGT1360.14392143582797157No Hit
CATAATACACATCTCCGAGCCCACGAGACGTTAACAGATCTCGTATGCCG1300.137571960717914No Hit
CTATACACATCTCCGAGCCCACGAGACGTTAACAGATCTCGTATGCCGTC1220.1291059939045039No Hit
CATATACACATCTCAGAGCCCACGAGACGTTAACAGATCTCGTATGCCGT1080.11429055198103623No Hit
CATATACACATCTCCGAGCCCACGAGACGTTAAAAGATCTCGTATGCCGT1060.11217406027768372No Hit
CATATACACATCTCCGAGACCACGAGACGTTAACAGATCTCGTATGCCGT1060.11217406027768372No Hit
CAAAAACACATCTCCGAGCCCACGAGACGTTAACAGATCTCGTATGCCGT1040.11005756857433119No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAACTCG500.070.0000136
ACAGAAC550.070.0000133
ATCTGCA455.456968E-1270.0000152
CCCTGAG150.002218570670.035
TCTGCCG150.002218570670.043
GACAATT150.002218570670.029
CTGAGGT150.002218570670.037
AAAAGTG150.002218570670.067
ATTACAC207.8910605E-570.02
AAAAAGT150.002218570670.066
AGCATCT150.002218570670.07
TACGAGA207.8910605E-570.013
TACTGAT150.002218570670.052
ACAGCTT150.002218570670.053
GAAGTCT150.002218570670.046
AGACAAT207.8910605E-570.028
GGTGCCC150.002218570670.045
GTCCCCC150.002218570670.031
TTGCACT150.002218570670.028
CACAAAT150.002218570670.07
Produced by FastQC (version 0.11.5)