FastQCFastQC Report
Wed 25 May 2016
SRR1781010_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1781010_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences254738
Sequences flagged as poor quality0
Sequence length76
%GC58

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG5543621.76196719766976No Hit
CTTATACACATCTGACGCTGCCGACGAACATAAGGGTGTAGATCTCGGTG11490.4510516687734064No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACACAGT308.353803E-658.34233535
GGTACAT308.3732775E-658.3194271
CGGTGGT1400.057.50887346
CTCGGTG1550.056.47141344
TGGTCGC1450.055.5040149
TCGGTGG1650.055.16002345
TCTCGGT1600.054.70668443
GTCGCCG1350.054.4314751
GCCGTAT1250.053.18732554
AGATCTC1450.053.11164540
ATTAACG200.006936225652.51841718
AACGTCC200.00694161652.50810246
GCATCGA200.00694161652.50810229
GATCTCG1550.051.95369741
GGTGGTC1700.051.46842647
TCGCCGT1350.049.2475252
TAGATCT1500.049.00755739
ATCTCGG1650.048.8049942
AGGTCGG305.866273E-446.67386625
CGCGTCT305.8776425E-446.655547
Produced by FastQC (version 0.11.5)