FastQCFastQC Report
Wed 25 May 2016
SRR1781010_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1781010_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences254738
Sequences flagged as poor quality0
Sequence length76
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CATATACACATCTCCGAGCCCACGAGACATCACTCGATCTCGTATGCCGT3236312.704425723684725No Hit
CTTATACACATCTCCGAGCCCACGAGACATCACTCGATCTCGTATGCCGT48551.9058797666622176No Hit
TCTTATACACATCTCCGAGCCCACGAGACATCACTCGATCTCGTATGCCG23170.9095619813298369No Hit
CACTTATACACATCTCCGAGCCCACGAGACATCACTCGATCTCGTATGCC21790.8553886738531354No Hit
CAAATACACATCTCCGAGCCCACGAGACATCACTCGATCTCGTATGCCGT18450.7242735673515533No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG18020.7073934787899724No Hit
CTCTTATACACATCTCCGAGCCCACGAGACATCACTCGATCTCGTATGCC7680.3014862329138173No Hit
AAACACATCTCCGAGCCCACGAGACATCACTCGATCTCGTATGCCGTCTT5580.21904859110144542RNA PCR Primer, Index 42 (95% over 22bp)
ACTTATACACATCTCCGAGCCCACGAGACATCACTCGATCTCGTATGCCG5230.20530898413271673No Hit
TCTTTATACACATCTCCGAGCCCACGAGACATCACTCGATCTCGTATGCC4150.16291248262921118No Hit
CAATACACATCTCCGAGCCCACGAGACATCACTCGATCTCGTATGCCGTC3480.1366109492890735No Hit
ATACACATCTCCGAGCCCACGAGACATCACTCGATCTCGTATGCCGTCTT2560.10049541097127246RNA PCR Primer, Index 42 (95% over 22bp)

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATCC150.00222310969.9984341
TCGTATC150.00222310969.9984340
CTCGAGG150.00222310969.998432
TCGAGGG150.00222310969.998433
CATATAC37650.064.79271
AGGGGGG45950.064.36270
ATATACA37950.064.280512
AACTCGT401.0628355E-861.24862737
TGCCGAC352.967172E-759.99865345
CAAATAC3700.056.755481
ATCGATC252.3877578E-455.9987433
ATCTCGT53800.055.10099437
CCGTCTT53700.054.9536947
CGTCTTC54650.054.89518448
CTCGTAT54550.054.856739
TCTCGTA54550.054.856738
TATGCCG53850.054.8548543
AAGGGGG53550.054.8353769
GATCTCG54350.054.8009836
ATGCCGT54050.054.7166344