FastQCFastQC Report
Wed 25 May 2016
SRR1781009_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1781009_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences196449
Sequences flagged as poor quality0
Sequence length76
%GC58

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG4300821.892704976864223No Hit
CTTATACACATCTGACGCTGCCGACGAACATAAGGGTGTAGATCTCGGTG9670.4922397161604284No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCCACT150.0022213870.0061137
CGGTGGT1700.055.59308646
GCCGTAT1450.055.5220954
GTCGCCG1400.055.00480351
CGTATCA1400.054.976856
TCTCGGT1750.054.0047243
TGGTCGC1500.053.6713549
CTCGGTG1700.053.53408444
TCGCCGT1450.053.10808652
GTCAGTA200.006934107352.5179630
ATTCCGG200.006934107352.5179621
TCGTTAC200.006941098352.5045816
CGGCCAT200.00695509752.4778558
GCTGACC200.00695509752.477858
ATTTCCG200.00695509752.4778548
TCGGTGG1750.052.00454345
CGCCGTA1550.051.9400253
ATCTCGG1600.050.3168942
CCGTATC1600.048.129255
GGTGGTC1800.046.67074247
Produced by FastQC (version 0.11.5)