FastQCFastQC Report
Wed 25 May 2016
SRR1781009_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1781009_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences196449
Sequences flagged as poor quality0
Sequence length76
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CATATACACATCTCCGAGCCCACGAGACATAAGTTAATCTCGTATGCCGT2208811.24363066241111No Hit
CAAATACACATCTCCGAGCCCACGAGACATAAGTTAATCTCGTATGCCGT26921.3703302129305825No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG15850.8068251810902575No Hit
CACTTATACACATCTCCGAGCCCACGAGACATAAGTTAATCTCGTATGCC15650.7966444217074151No Hit
CTTATACACATCTCCGAGCCCACGAGACATAAGTTAATCTCGTATGCCGT10670.5431435130746403No Hit
TCTTATACACATCTCCGAGCCCACGAGACATAAGTTAATCTCGTATGCCG9940.5059837413272655No Hit
CATATACAAATCTCCGAGCCCACGAGACATAAGTTAATCTCGTATGCCGT5630.2865883766270126No Hit
ACTTATACACATCTCCGAGCCCACGAGACATAAGTTAATCTCGTATGCCG5300.26979012364532273No Hit
AAACACATCTCCGAGCCCACGAGACATAAGTTAATCTCGTATGCCGTCTT5080.2585912883241961RNA PCR Primer, Index 15 (95% over 21bp)
CAATACACATCTCCGAGCCCACGAGACATAAGTTAATCTCGTATGCCGTC3760.1913982763974365No Hit
TCTTTATACACATCTCCGAGCCCACGAGACATAAGTTAATCTCGTATGCC3040.15474754261920398No Hit
CATAAACACATCTCCGAGCCCACGAGACATAAGTTAATCTCGTATGCCGT2690.13693121369922984No Hit
ATACACATCTCCGAGCCCACGAGACATAAGTTAATCTCGTATGCCGTCTT2670.13591313776094557RNA PCR Primer, Index 15 (95% over 21bp)
CATATACACATCTCCGAGCCCACGAGACATAAGTTAATCTCGTATGACGT2610.1328589099460929No Hit
CATATACACATCTCCGAGCCCACGAGACATAAGATAATCTCGTATGCCGT2570.1308227580695244No Hit
TCATATACACATCTCCGAGCCCACGAGACATAAGTTAATCTCGTATGCCG2470.12573237837810322No Hit
CATATACACATCTCCGAGCCCACGAGACATAAGTTAATATCGTATGCCGT2190.1114793152421239No Hit
CATTACACATCTCCGAGCCCACGAGACATAAGTTAATCTCGTATGCCGTC2020.1028256697667079No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTCGGTC150.002220708470.0114628
TCGGTCA150.002220708470.0114629
AAGTACG150.002222952769.993641
AGCCGAC150.002222952769.9936445
CATATAC27800.064.0769041
AGGGGGG30550.062.6780270
ATATACA28350.062.2165642
CGTCTAC850.061.7590948
CAAATAC6650.061.0470771
AAACTCG650.059.2404736
ATCTCGA850.057.65649437
TCGAATG1000.056.0091740
CACGTAT452.6846465E-854.45335839
AACTCGT653.6379788E-1253.85497337
AATCTCG41950.053.57253336
ATCCGAG1700.053.52454412
AATGCCG850.053.52454443
TGCCGTC40950.053.32848445
ATGCCGT41100.053.30415344
GCCGTCT40200.053.1916846
Produced by FastQC (version 0.11.5)