FastQCFastQC Report
Wed 25 May 2016
SRR1781008_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1781008_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences302313
Sequences flagged as poor quality0
Sequence length76
%GC58

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG6669222.060579597966345No Hit
CTTATACACATCTGACGCTGCCGACGAACATAAGGGTGTAGATCTCGGTG13000.43001789536010687No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CACGTCT150.00222535769.9837957
GTAGGTC200.00693983452.513925
CCGATAG200.00695347152.4878469
CGTGAGT305.869617E-446.671355
TTGCGAG305.879199E-446.655869
ACACTTG350.001252668940.0105929
GAGACGC350.001254709439.99735313
TGCAGCG350.001254709439.99735315
GCCGAGT350.001255730639.99073858
ACGTCTG350.001255730639.9907388
CGGTGGT1850.037.82907546
TGGTCGC1950.037.6898149
GCCGTAT1900.036.8457654
TCGCCGT2000.036.75364752
GATCTCG1950.035.90141
GACGAAA400.002413485235.0092725
TAAACAG501.7048517E-435.00926637
GTCGCCG2100.035.00347551
GAGCCGG501.7065112E-435.00347522
GCTCCCG501.7065112E-435.00347550
Produced by FastQC (version 0.11.5)