FastQCFastQC Report
Wed 25 May 2016
SRR1781008_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1781008_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences302313
Sequences flagged as poor quality0
Sequence length76
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CATATACACATCTCCGAGCCCACGAGACTTGCTAAGATCTCGTATGCCGT3815412.620694445822709No Hit
CTTATACACATCTCCGAGCCCACGAGACTTGCTAAGATCTCGTATGCCGT61402.031007598085428No Hit
TCTTATACACATCTCCGAGCCCACGAGACTTGCTAAGATCTCGTATGCCG29100.9625785196137777No Hit
CAAATACACATCTCCGAGCCCACGAGACTTGCTAAGATCTCGTATGCCGT23350.7723782966660382No Hit
CACTTATACACATCTCCGAGCCCACGAGACTTGCTAAGATCTCGTATGCC23330.7717167306731765No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG21630.7154836212799318No Hit
CTCTTATACACATCTCCGAGCCCACGAGACTTGCTAAGATCTCGTATGCC8860.2930737348377344No Hit
AAACACATCTCCGAGCCCACGAGACTTGCTAAGATCTCGTATGCCGTCTT6940.22956339952301094Illumina PCR Primer Index 9 (95% over 21bp)
ACTTATACACATCTCCGAGCCCACGAGACTTGCTAAGATCTCGTATGCCG5840.19317726991561726No Hit
TCTTTATACACATCTCCGAGCCCACGAGACTTGCTAAGATCTCGTATGCC5620.1859000439941385No Hit
CAATACACATCTCCGAGCCCACGAGACTTGCTAAGATCTCGTATGCCGTC4690.15513722532606933No Hit
ATACACATCTCCGAGCCCACGAGACTTGCTAAGATCTCGTATGCCGTCTT3890.12867458561160122Illumina PCR Primer Index 9 (95% over 21bp)
CATATACAAATCTCCGAGCCCACGAGACTTGCTAAGATCTCGTATGCCGT3230.10684290784716502No Hit
CATATACACATCTCCGAGCCCACGAGACTTGCTAAGATCTCGTATGACGT3090.10221194589713312No Hit
CATAAACACATCTCCGAGCCCACGAGACTTGCTAAGATCTCGTATGCCGT3090.10221194589713312No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAATGCG150.002222584870.0057946
CCGACTT500.070.0057947
GCCGTAT207.9159385E-570.0057846
GAAGTGC150.002224043769.994216
CATATAC43750.067.274441
ATATACA43900.067.044572
TGCCGAC600.064.17197445
AGGGGGG58300.063.87121670
ACTTGCA550.063.65215727
GCCGACT550.063.64162446
CGTCTTA501.4551915E-1163.00520748
AAGCCGT501.4551915E-1163.00520744
AGCCGTC453.8380676E-1062.22736745
GTATTCT401.0630174E-861.25506249
CACGTAT750.060.67168839
CAGCTTG1100.060.4595454
CGTCTAC700.060.00496348
ACGTATG650.059.23567240
CAAATAC5750.059.0385971
CGAATGC601.8189894E-1258.33815841