FastQCFastQC Report
Wed 25 May 2016
SRR1781007_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1781007_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences141989
Sequences flagged as poor quality0
Sequence length76
%GC71

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG5327237.518399312622805No Hit
CTTATACACATCTGACGCTGCCGACGAACATAAGGGTGTAGATCTCGGTG10570.7444238638204368No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGTCATC150.002222430269.987679
TAGCCTT150.002222430269.9876713
TCGACCA200.00693471752.5092525
GCTCATT200.00693471752.5092567
ACTATCC200.0069443952.4907534
TTGTGCG200.0069443952.4907532
GGGCTAA200.0069443952.4907531
TTCACAT200.0069443952.49075353
CTGCGAC200.0069443952.49075349
TAATGGA200.0069443952.4907534
TTGTAAG305.8670004E-446.65844757
CGTATCA1550.045.15333656
CGCCGTA1650.044.5376153
ATCATTA1800.040.82614559
TCTCGGT1900.040.51917643
CCGTATC1750.039.99295455
GTATCAT1750.039.99295457
GCCGTAT1750.039.99295454
GGTCGCC1850.039.72273350
GTAGATC1950.039.49413738
Produced by FastQC (version 0.11.5)