FastQCFastQC Report
Wed 25 May 2016
SRR1781007_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1781007_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences141989
Sequences flagged as poor quality0
Sequence length76
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CATATACACATCTCCGAGCCCACGAGACATTGCCGGATCTCGTATGCCGT2905120.460035636563394No Hit
CTTATACACATCTCCGAGCCCACGAGACATTGCCGGATCTCGTATGCCGT43433.058687644817556No Hit
TCTTATACACATCTCCGAGCCCACGAGACATTGCCGGATCTCGTATGCCG23161.6311122692602948No Hit
CACTTATACACATCTCCGAGCCCACGAGACATTGCCGGATCTCGTATGCC18251.2853108339378403No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG17941.2634781567586222No Hit
CAAATACACATCTCCGAGCCCACGAGACATTGCCGGATCTCGTATGCCGT16111.1345949334103347No Hit
CTCTTATACACATCTCCGAGCCCACGAGACATTGCCGGATCTCGTATGCC6010.42327222531322845No Hit
ACTTATACACATCTCCGAGCCCACGAGACATTGCCGGATCTCGTATGCCG4690.33030727732429976No Hit
CATATACACATCTCCGAGCCCACGAGACATTGCCGGATCTCGTATGACGT4480.31551739923515204No Hit
AAACACATCTCCGAGCCCACGAGACATTGCCGGATCTCGTATGCCGTCTT4300.3028403608730254RNA PCR Primer, Index 30 (95% over 22bp)
CATATACACATCTCCGAGCCCACGAGACATTGCAGGATCTCGTATGCCGT3530.24861080787948364No Hit
TCTTTATACACATCTCCGAGCCCACGAGACATTGCCGGATCTCGTATGCC3420.24086372888040622No Hit
CAATACACATCTCCGAGCCCACGAGACATTGCCGGATCTCGTATGCCGTC3360.236638049426364No Hit
CATATACACATCTCCGAGCCCACGAGACATTGCCGGATCTAGTATGCCGT3070.21621393206515999No Hit
CATATACACATCTCCGAGCCCACGAGACATTGACGGATCTCGTATGCCGT2540.1788870968877871No Hit
ATACACATCTCCGAGCCCACGAGACATTGCCGGATCTCGTATGCCGTCTT2440.17184429779771673RNA PCR Primer, Index 30 (95% over 22bp)
CATTACACATCTCCGAGCCCACGAGACATTGCCGGATCTCGTATGCCGTC2110.14860306080048455No Hit
CATAAACACATCTCCGAGCCCACGAGACATTGCCGGATCTCGTATGCCGT2070.1457859411644564No Hit
TCATATACACATCTCCGAGCCCACGAGACATTGCCGGATCTCGTATGCCG2060.14508166125544936No Hit
CATATACAAATCTCCGAGCCCACGAGACATTGCCGGATCTCGTATGCCGT1880.13240462289332272No Hit
CATTATACACATCTCCGAGCCCACGAGACATTGCCGGATCTCGTATGCCG1620.11409334525913979No Hit
CATATACACATCTCCGAGCCCACGAGACATTGCCGGATCTCGTAAGCCGT1570.11057194571410461No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCACGT207.904737E-570.0000137
ATCGTAA207.904737E-570.0000139
AACGAGC207.904737E-570.0000113
ATCTCGA500.070.037
CGAACGC150.002220878170.020
TTATATC150.002220878170.054
GTAGAGC150.002220878170.03
CGTAAGA353.6816346E-970.041
CCTATGC150.002220878170.041
CACTTAT2150.068.372091
CATATAC36050.068.2524261
ATATACA36100.067.770082
CAAATAC4650.065.483871
AGGGGGG44550.065.050570
CCGTATT1150.063.9130447
TCGTAAG1500.063.040
TGCCGTA1500.063.045
ATATCGT950.062.63158437
CCGTAAT453.8198777E-1062.22222547
GCCGTAT1150.060.86956446
Produced by FastQC (version 0.11.5)