FastQCFastQC Report
Wed 25 May 2016
SRR1781006_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1781006_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences310134
Sequences flagged as poor quality0
Sequence length76
%GC57

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG6471720.867431497352758No Hit
CTTATACACATCTGACGCTGCCGACGAACATAAGGGTGTAGATCTCGGTG19230.6200545570624311No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CAGTAGT150.002223692869.997421
TGATAAC200.00694385652.5065325
GACCTTC200.00694385652.5065316
GAGTTAT200.006952719852.48967
ACGTACT305.8737927E-446.6649540
TCGCCGT3300.045.61173652
GCCGTAT3300.045.61173654
CGTATCA3500.044.9983456
CGGTGGT3750.043.8579846
GACATCG404.5836394E-543.75544422
TACGTTT404.5836394E-543.75544416
GCTAGTG404.592377E-543.7413333
GTATCAT3550.043.3716957
TGGTCGC3650.042.19022849
GTACATG503.4735222E-641.998451
TGTGCGC503.4773766E-641.991683
TGCGCGT503.4773766E-641.991685
TCGGTGG4000.041.9916845
TCTCGGT3850.041.809943
AGATCTC3700.041.62008740
Produced by FastQC (version 0.11.5)