FastQCFastQC Report
Wed 25 May 2016
SRR1781006_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1781006_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences310134
Sequences flagged as poor quality0
Sequence length76
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CATATACACATCTCCGAGCCCACGAGACAGTAGATTATCTCGTATGCCGT3725312.01190453158957No Hit
CTTATACACATCTCCGAGCCCACGAGACAGTAGATTATCTCGTATGCCGT63352.0426654284922003No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG30000.9673238019694712No Hit
TCTTATACACATCTCCGAGCCCACGAGACAGTAGATTATCTCGTATGCCG29780.9602300940883619No Hit
CACTTATACACATCTCCGAGCCCACGAGACAGTAGATTATCTCGTATGCC22800.7351660894967982No Hit
CAAATACACATCTCCGAGCCCACGAGACAGTAGATTATCTCGTATGCCGT21320.6874447819329709No Hit
CTCTTATACACATCTCCGAGCCCACGAGACAGTAGATTATCTCGTATGCC9330.30083770241250557No Hit
ACTTATACACATCTCCGAGCCCACGAGACAGTAGATTATCTCGTATGCCG6780.2186151792451005No Hit
AAACACATCTCCGAGCCCACGAGACAGTAGATTATCTCGTATGCCGTCTT5290.1705714304139501Illumina PCR Primer Index 10 (95% over 23bp)
TCTTTATACACATCTCCGAGCCCACGAGACAGTAGATTATCTCGTATGCC4960.15993086859228592No Hit
CATATACACATCTCCGAGCCCACGAGACAGTAGATTATCTCGTATGACGT4050.13058871326587862No Hit
CAATACACATCTCCGAGCCCACGAGACAGTAGATTATCTCGTATGCCGTC3810.12285012285012285Illumina PCR Primer Index 10 (95% over 21bp)
ATACACATCTCCGAGCCCACGAGACAGTAGATTATCTCGTATGCCGTCTT3340.10769538328593448Illumina PCR Primer Index 10 (95% over 23bp)
CATAAACACATCTCCGAGCCCACGAGACAGTAGATTATCTCGTATGCCGT3330.10737294201861132No Hit
CATATACAAATCTCCGAGCCCACGAGACAGTAGATTATCTCGTATGCCGT3230.10414852934537974No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACCGGCC150.002222657470.00564646
TACGTCA150.002222657470.00564657
CATATAC43900.066.646111
ATATACA44350.065.890972
AGGGGGG57450.064.63360670
AATCTCG650.064.620636
CAAATAC6250.059.3552171
AAGGGGG65900.056.08045669
AAAACGG252.3892579E-455.99548367
CGAGAAC252.3892579E-455.99548315
CCGTCTT63700.055.77372447
GCCGTCT64150.055.70987346
CGTCTTC64750.055.62610648
TATGCCG66600.055.49996643
ATGCCGT66850.055.34477244
TGCCGTC66600.055.34229745
GTATGCC67150.055.09751542
CGTATGC67900.055.05598441
GTCTTCT64700.055.0198949
TCTTCTG64600.054.98782350