FastQCFastQC Report
Wed 25 May 2016
SRR1781005_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1781005_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences295616
Sequences flagged as poor quality0
Sequence length76
%GC57

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG5791319.590617557912967No Hit
CTTATACACATCTGACGCTGCCGACGAACATAAGGGTGTAGATCTCGGTG11220.3795464386230786No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCGCCT150.002225567669.9817363
CGGTGGT1950.057.4209146
TGGTCGC1900.057.1096849
GCCGTAT1800.056.39325354
CTCGGTG2100.054.9856544
TCGGTGG2100.054.9856545
TCGCCGT1950.053.8503252
CGTATCA1900.053.40711256
CGTCGTT200.006940181452.51294730
CGATAGG200.006940181452.51294718
GCGATCA200.006944827752.50406315
GAGTCTA200.006954128452.4862981
GGGTCTA200.006954128452.48629810
CAGTTGT200.006954128452.4862981
GTGGTAT200.006954128452.48629858
GTCGCCG2100.051.67066251
AGATCTC2150.050.4690240
CGCCGTA2100.050.0038753
ATCTCGG2200.049.32199542
GATCTCG2200.049.32199541
Produced by FastQC (version 0.11.5)