FastQCFastQC Report
Wed 25 May 2016
SRR1781005_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1781005_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences295616
Sequences flagged as poor quality0
Sequence length76
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CATATACACATCTCCGAGCCCACGAGACGTGATAGCATCTCGTATGCCGT3252611.002787399870103No Hit
CTTATACACATCTCCGAGCCCACGAGACGTGATAGCATCTCGTATGCCGT60922.060781554449015No Hit
TCTTATACACATCTCCGAGCCCACGAGACGTGATAGCATCTCGTATGCCG25820.8734303961896515No Hit
CACTTATACACATCTCCGAGCCCACGAGACGTGATAGCATCTCGTATGCC21670.7330455726347694No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG18440.6237822039402467No Hit
CAAATACACATCTCCGAGCCCACGAGACGTGATAGCATCTCGTATGCCGT16930.5727024247672656No Hit
CTCTTATACACATCTCCGAGCCCACGAGACGTGATAGCATCTCGTATGCC8190.2770486035938515No Hit
AAACACATCTCCGAGCCCACGAGACGTGATAGCATCTCGTATGCCGTCTT5270.1782718120805369RNA PCR Primer, Index 24 (95% over 23bp)
ACTTATACACATCTCCGAGCCCACGAGACGTGATAGCATCTCGTATGCCG5070.17150627841524138No Hit
TCTTTATACACATCTCCGAGCCCACGAGACGTGATAGCATCTCGTATGCC4950.1674469582160641No Hit
CAATACACATCTCCGAGCCCACGAGACGTGATAGCATCTCGTATGCCGTC3820.1292216930071444RNA PCR Primer, Index 24 (95% over 21bp)
CATATACACATCTCCGAGCCCACGAGACGTGATAGCATCTCGTATGACGT3360.11366096557696471No Hit
ATACACATCTCCGAGCCCACGAGACGTGATAGCATCTCGTATGCCGTCTT3230.10926336869452262RNA PCR Primer, Index 24 (95% over 23bp)
CATATACAAATCTCCGAGCCCACGAGACGTGATAGCATCTCGTATGCCGT3000.10148300497943277No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCGTAA150.002222455670.0064339
TCGAAAG150.002222455670.0064340
CCGACGA150.002223947369.9945821
CCTATCG150.002223947369.9945815
ATATACA39900.065.7843862
CATATAC40000.065.532431
AGGGGGG50600.065.16408570
CCGACTT800.061.24526647
CAACTCG601.8189894E-1258.3386936
CTCTCGT308.360212E-658.3386937
TGCCGAC900.058.3288245
TCTTCTG54850.057.1058950
AAGGGGG57900.057.06914569
CGTCTTC55650.056.85095248
ACGAGAT1050.056.67186727
CCGTCTT55450.056.61419747
GTCTTCT55300.056.5146249
GCCGTCT55750.056.4978746
TATGCCG57150.056.46107543
CTTCTGC56100.056.45730651