FastQCFastQC Report
Wed 25 May 2016
SRR1781004_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1781004_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences304449
Sequences flagged as poor quality0
Sequence length76
%GC60

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG7730825.392758721493585No Hit
CTTATACACATCTGACGCTGCCGACGAACATAAGGGTGTAGATCTCGGTG13160.4322563056538205No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGAAGGA150.00222169470.0129825
TGCGCAT150.002224591669.989985
TCGCCGT2950.055.7547352
GCCGTAT2950.053.38218754
AGATCTC3000.052.50973540
GATCTCG3000.052.5011141
CAGTATT200.00695108652.4924851
CCACGTA407.473391E-752.49248560
TGGTCGC3200.052.49248549
GTAGATC2950.052.2216538
GTCGCCG3150.052.2147551
CGGTGGT3150.052.2147546
CTCGGTG3100.051.9280544
TCGGTGG3400.050.43395245
ATCTCGG3150.049.99284442
GCGCGTC352.0855912E-549.9928446
CGCGTCT352.0855912E-549.9928447
CGCCGTA3300.049.84134753
TCTCGGT3200.049.21170443
TAGATCT3200.048.13392339
Produced by FastQC (version 0.11.5)