FastQCFastQC Report
Wed 25 May 2016
SRR1781004_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1781004_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences304449
Sequences flagged as poor quality0
Sequence length76
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CATATACACATCTCCGAGCCCACGAGACTACTTGAAATCTCGTATGCCGT4563414.9890457843514No Hit
CTTATACACATCTCCGAGCCCACGAGACTACTTGAAATCTCGTATGCCGT58341.9162486984683806No Hit
TCTTATACACATCTCCGAGCCCACGAGACTACTTGAAATCTCGTATGCCG31841.0458237668706416No Hit
CACTTATACACATCTCCGAGCCCACGAGACTACTTGAAATCTCGTATGCC27110.8904611281364038No Hit
CAAATACACATCTCCGAGCCCACGAGACTACTTGAAATCTCGTATGCCGT26860.8822495721779346No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG21370.7019238033299502No Hit
AAACACATCTCCGAGCCCACGAGACTACTTGAAATCTCGTATGCCGTCTT8230.2703244221528072RNA PCR Primer, Index 12 (95% over 23bp)
ACTTATACACATCTCCGAGCCCACGAGACTACTTGAAATCTCGTATGCCG8110.26638287529274196No Hit
CTCTTATACACATCTCCGAGCCCACGAGACTACTTGAAATCTCGTATGCC7950.2611274794793217No Hit
TCTTTATACACATCTCCGAGCCCACGAGACTACTTGAAATCTCGTATGCC5540.18196808003967824No Hit
CAATACACATCTCCGAGCCCACGAGACTACTTGAAATCTCGTATGCCGTC5450.17901191989462933TruSeq Adapter, Index 12 (95% over 21bp)
CATATACAAATCTCCGAGCCCACGAGACTACTTGAAATCTCGTATGCCGT4720.15503417649589915No Hit
CATATACACATCTCCGAGCCCACGAGACTACTTGAAATCTCGTATGACGT4000.13138489533550773No Hit
ATACACATCTCCGAGCCCACGAGACTACTTGAAATCTCGTATGCCGTCTT3320.10904946312847144TruSeq Adapter, Index 12 (95% over 23bp)
CATTACACATCTCCGAGCCCACGAGACTACTTGAAATCTCGTATGCCGTC3230.10609330298342251No Hit
CATAAACACATCTCCGAGCCCACGAGACTACTTGAAATCTCGTATGCCGT3160.10379406731505113No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTTTGAC150.002223556969.9981928
AACGTGA150.002223556969.998191
GGATAGA252.8364102E-669.998191
CGTAATC150.002223556969.9981948
GCCGTAA150.002223556969.9981946
CATATAC55300.067.2134551
ATATACA55650.066.853622
AGGGGGG69850.064.6975870
CTTTATA1550.063.224182
CAAATAC7000.061.9983981
TGCCGTA401.0639269E-861.24841745
ACGTATG750.060.6750740
AAACTCG950.058.95553636
CGACTTA308.366642E-658.33182548
CGCAAAG308.366642E-658.33182512
CAACTTG850.057.6455728
AAGGGGG78750.057.20792469
AACTCGT800.056.88287737
CTGCTTG78600.056.3280654
TATGCCG80000.056.26104743
Produced by FastQC (version 0.11.5)