FastQCFastQC Report
Wed 25 May 2016
SRR1781003_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1781003_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences283433
Sequences flagged as poor quality0
Sequence length76
%GC59

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG6397922.572883185796996No Hit
CTTATACACATCTGACGCTGCCGACGAACATAAGGGTGTAGATCTCGGTG10720.3782198967657259No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGACGAT150.002222369770.0063522
TTGACAT200.006944164652.5047742
AACATGC200.00695386552.4862461
TTAAGTA305.874347E-446.66266619
TACGGAG305.87946E-446.6544345
TTGGGCG305.87946E-446.6544342
GCATAGG305.87946E-446.6544343
CGGTGGT2550.042.5378746
TCGGTGG2500.041.9889945
CTCGGTG2450.041.4250244
TGGTCGC2250.040.4409849
GCCGTAT2000.040.2465554
GGTCTCG350.001252520540.01069341
GTCTCGG350.001253608440.00362842
TCGCCGT2100.039.99657452
GCCTTTA350.001255786439.9895131
CTACGGA350.001255786439.9895134
TCTCGGT2700.038.88555543
AGATCTC2200.038.19202440
GATCTCG2200.038.19202441
Produced by FastQC (version 0.11.5)