FastQCFastQC Report
Wed 25 May 2016
SRR1781003_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1781003_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences283433
Sequences flagged as poor quality0
Sequence length76
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CATATACACATCTCCGAGCCCACGAGACACAAAGTGATCTCGTATGCCGT3553612.537707324129514TruSeq Adapter, Index 5 (95% over 23bp)
CTTATACACATCTCCGAGCCCACGAGACACAAAGTGATCTCGTATGCCGT62392.20122568649381TruSeq Adapter, Index 5 (95% over 23bp)
TCTTATACACATCTCCGAGCCCACGAGACACAAAGTGATCTCGTATGCCG26320.9286145226561481TruSeq Adapter, Index 5 (95% over 22bp)
CAAATACACATCTCCGAGCCCACGAGACACAAAGTGATCTCGTATGCCGT20810.7342123182551079TruSeq Adapter, Index 5 (95% over 23bp)
CACTTATACACATCTCCGAGCCCACGAGACACAAAGTGATCTCGTATGCC20120.709867940571493TruSeq Adapter, Index 5 (95% over 21bp)
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG18510.6530643926430585No Hit
CTCTTATACACATCTCCGAGCCCACGAGACACAAAGTGATCTCGTATGCC8550.3016585930360967RNA PCR Primer, Index 5 (95% over 21bp)
AAACACATCTCCGAGCCCACGAGACACAAAGTGATCTCGTATGCCGTCTT6720.23709306961433568RNA PCR Primer, Index 5 (96% over 26bp)
TCTTTATACACATCTCCGAGCCCACGAGACACAAAGTGATCTCGTATGCC5420.19122685079013382RNA PCR Primer, Index 5 (95% over 21bp)
ACTTATACACATCTCCGAGCCCACGAGACACAAAGTGATCTCGTATGCCG5260.18558177770407822No Hit
CAATACACATCTCCGAGCCCACGAGACACAAAGTGATCTCGTATGCCGTC4940.17429163153196697TruSeq Adapter, Index 5 (95% over 24bp)
ATACACATCTCCGAGCCCACGAGACACAAAGTGATCTCGTATGCCGTCTT3880.13689302233684855TruSeq Adapter, Index 5 (96% over 26bp)
CATATACACATCTCCGAGCCCACGAGACACAAAGTGATCTCGTATGACGT3330.1174880836035324No Hit
CATATACAAATCTCCGAGCCCACGAGACACAAAGTGATCTCGTATGCCGT3160.11149019344959832No Hit
CATTACACATCTCCGAGCCCACGAGACACAAAGTGATCTCGTATGCCGTC2940.10372821795627185No Hit
CATAAACACATCTCCGAGCCCACGAGACACAAAGTGATCTCGTATGCCGT2840.1002000472774871No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACACGTC150.002223503869.9973517
CGAGTTG150.002223503869.9973545
GCAAACG150.002223503869.9973518
TGACGTA150.002223503869.9973545
TGACGAC207.919812E-569.9973545
CCGACTT401.3460522E-1069.9973547
CGTAATC252.8361064E-669.9973548
TTCGGCC150.002223503869.9973543
CACGTCT150.002223503869.9973518
CATATAC43250.065.072611
ATATACA43050.065.0381852
AGGGGGG53550.063.9845170
CAAATAC5200.063.2780041
AACTCGT501.4551915E-1162.9976237
CACGTAT1900.062.6292139
ACGTATG2050.061.46109440
TCACGTA2000.059.4977538
CACGAAT308.365754E-658.33112739
CTTTATA1450.057.9288442
ATCTGCA800.056.8828952
Produced by FastQC (version 0.11.5)