FastQCFastQC Report
Wed 25 May 2016
SRR1781002_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1781002_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences203238
Sequences flagged as poor quality0
Sequence length76
%GC64

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG6269630.848561784705613No Hit
CTTATACACATCTGACGCTGCCGACGAGGCAACCTGTGTAGATCTCGGTG18700.9201035239472932No Hit
ATACACATCTGACGCTGCCGACGAGGCAACCTGTGTAGATCTCGGTGGTC2450.1205483226561962No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGAGCG150.002220494770.0140333
GAGCGAA150.002220494770.0140335
TGCGCGT252.8374943E-669.9795765
ACTGGCG150.002224835369.97957643
AATGCGA252.3781788E-456.03881525
ACTCGCT252.3868777E-455.9974450
GCACTCG200.006924834652.5363925
GCGAAAG200.00693833952.51052537
TACGGGC200.00693833952.51052539
ACGGGCT200.00693833952.51052540
CTACGGG200.00693833952.51052538
AGCGAAA200.00693833952.51052536
CGTGGAA200.006945098852.497617
AGCACCT200.00695186352.4846810
ATCTTCC200.00695186352.484689
GCCGTAT3800.048.81355754
CGGTGGT3950.047.8341446
GTCGCCG3900.047.5619351
TCGCCGT3850.047.2705752
CTCGGTG3850.047.25893444
Produced by FastQC (version 0.11.5)