FastQCFastQC Report
Wed 25 May 2016
SRR1781002_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1781002_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences203238
Sequences flagged as poor quality0
Sequence length76
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTTTCGTCAATCTCGTATGCCGT4034019.848650350820222No Hit
TCTTATACACATCTCCGAGCCCACGAGACTTTCGTCAATCTCGTATGCCG44552.1920113364626697No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG31531.551383107489741No Hit
CTCTTATACACATCTCCGAGCCCACGAGACTTTCGTCAATCTCGTATGCC23161.139550674578573No Hit
CATATACACATCTCCGAGCCCACGAGACTTTCGTCAATCTCGTATGCCGT22301.0972357531563979No Hit
ATACACATCTCCGAGCCCACGAGACTTTCGTCAATCTCGTATGCCGTCTT17390.8556470738739802RNA PCR Primer, Index 48 (95% over 23bp)
TCTTTATACACATCTCCGAGCCCACGAGACTTTCGTCAATCTCGTATGCC9570.47087650931420305No Hit
CTATACACATCTCCGAGCCCACGAGACTTTCGTCAATCTCGTATGCCGTC7010.34491581298772866No Hit
CTTTACACATCTCCGAGCCCACGAGACTTTCGTCAATCTCGTATGCCGTC4690.2307639319418613RNA PCR Primer, Index 48 (95% over 21bp)
CTTTATACACATCTCCGAGCCCACGAGACTTTCGTCAATCTCGTATGCCG3040.14957832688768832No Hit
CTTATACACATCTCCGAGCCCACGAGACTTTCGTCAATCTCGTATGACGT2820.13875357954713194No Hit
CTTATACACATCTCCGAGCCCACGAGACTTTCGTCAATATCGTATGCCGT2730.13432527381690432No Hit
CTTAATACACATCTCCGAGCCCACGAGACTTTCGTCAATCTCGTATGCCG2490.12251645853629735No Hit
CTTATACACATCTCCGAGCCCACGAGACTATCGTCAATCTCGTATGCCGT2420.11907222074612031No Hit
TATACACATCTCCGAGCCCACGAGACTTTCGTCAATCTCGTATGCCGTCT2140.10529526958541217RNA PCR Primer, Index 48 (95% over 22bp)
CTTATACACATCTCCGAGCCCACGAGACTTTCGTCATCTCGTATGCCGTC2060.10135899782520985No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAACGTA150.002218916570.0265938
TCGTCTA150.002221084770.0093531
CTTTCGA455.456968E-1270.0093528
ACGCGGC150.002223254269.9921322
AGGGGGG50250.062.48596270
CGAATGC750.060.67477441
CGTCTTA308.348774E-658.34112548
TTCGTAA308.348774E-658.34112530
TCGAATG850.057.66895340
TCTTTAT1900.057.098841
TACGTCA252.384764E-456.0074830
CGACAAT509.767973E-1055.99370232
CGTATGA900.054.45171741
GTATGAC850.053.53656442
AAGGGGG59550.052.72744469
ACGTATG200.006933420452.5199440
AGCCGTC407.4467425E-752.50701545
GGTCACC200.006940175352.50701531
CCGCTGT200.006940175352.50701569
ACTTACG200.006940175352.50701527