FastQCFastQC Report
Wed 25 May 2016
SRR1781001_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1781001_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences390706
Sequences flagged as poor quality0
Sequence length76
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG4639711.875169564839034No Hit
CTTATACACATCTGACGCTGCCGACGAGGCAACCTGTGTAGATCTCGGTG11970.3063684714337635No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CAGTTAG150.002221948270.0147220
ACACGTT308.365703E-658.33813515
GCCGGAC200.006946359352.5043225
GGTCGAG200.006949876452.497611
CGCCGTG200.006949876452.497613
ACGTTCC352.0839081E-550.00411617
CGTTCCC352.0839081E-550.00411618
GGGTCTA305.872231E-446.6705061
GCCGTAT2300.044.13406454
TCGCCGT2500.040.6033452
TGGTCGC2450.039.99817349
GTCGCCG2650.039.625951
CGGTGGT2500.039.1982146
TCGGTGG2600.039.03667445
CTCGGTG2650.038.30013744
ATCTCGG2850.036.8404242
AGATCTC2850.036.8404240
TCTCGGT2900.036.20524243
GATCTCG2950.035.5915941
TGATCGT501.7075948E-435.00288415
Produced by FastQC (version 0.11.5)