FastQCFastQC Report
Wed 25 May 2016
SRR1781001_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1781001_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences390706
Sequences flagged as poor quality0
Sequence length76
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACGAGCACGTATCTCGTATGCCGT283897.266077306209784No Hit
TCTTATACACATCTCCGAGCCCACGAGACGAGCACGTATCTCGTATGCCG34580.8850644730308723No Hit
CTCTTATACACATCTCCGAGCCCACGAGACGAGCACGTATCTCGTATGCC21400.5477264234488337No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG20900.5349290771065712No Hit
CATATACACATCTCCGAGCCCACGAGACGAGCACGTATCTCGTATGCCGT17830.45635337056507963No Hit
ATACACATCTCCGAGCCCACGAGACGAGCACGTATCTCGTATGCCGTCTT12320.3153266138733472TruSeq Adapter, Index 2 (95% over 21bp)
TCTTTATACACATCTCCGAGCCCACGAGACGAGCACGTATCTCGTATGCC7560.19349587669500853No Hit
CTATACACATCTCCGAGCCCACGAGACGAGCACGTATCTCGTATGCCGTC5240.13411618966691066No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGGGGGG38250.061.57933470
CGTCTAC553.6379788E-1157.2778648
AAGTATC750.055.9978533
TGACGCG200.006946157752.50470437
AACTCGT800.052.50470437
TTACACG200.006946157752.50470443
TGCCGGA200.006946157752.50470446
AACGTAT407.4735E-752.49798232
TTCCGCG200.00694967552.4979822
TTAAGCA200.00694967552.49798252
GGTTACA200.00694967552.4979821
CTTTATA1650.050.907142
CGTCTTC47300.050.02562348
CTTGAAA48100.049.98768257
CTCGTAT49750.049.8838739
TCGTATG49750.049.8838740
GCCGTCT47100.049.86646346
CCGTCTT46650.049.82225447
TATGCCG47850.049.81637643
CGTATGC48800.049.77905341