FastQCFastQC Report
Wed 25 May 2016
SRR1781000_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1781000_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences58620
Sequences flagged as poor quality0
Sequence length76
%GC50

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG600310.240532241555783No Hit
CTTATACACATCTGACGCTGCCGACGAGGCAACCTGTGTAGATCTCGGTG3860.6584783350392358No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCCCCGG150.002214354570.068
GGTATAC150.002214354570.01
CGGTGGT800.056.87546
CTCGGTG750.056.044
GCCGTAT750.056.054
TGGTCGC750.056.049
TCGCCGT700.055.052
ATCTCGG700.055.042
AGATCTC700.055.040
TCTCGGT700.055.043
GATCTCG700.055.041
TCGGTGG850.053.5294145
CCTGGAG200.006919324452.53
GCTTTAG200.006919324452.51
GTCGCCG750.051.33333251
CTGTGTA750.051.33333234
CCGTATC850.049.41176655
CGCCGTA850.049.41176653
GTAGATC800.048.12538
CGTATCA900.046.66666856
Produced by FastQC (version 0.11.5)