FastQCFastQC Report
Wed 25 May 2016
SRR1781000_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1781000_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences58620
Sequences flagged as poor quality0
Sequence length76
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACAATGGTAGATCTCGTATGCCGT14912.5435005117707266No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG7971.3596042306380074No Hit
ATACACATCTCCGAGCCCACGAGACAATGGTAGATCTCGTATGCCGTCTT5210.88877516206073RNA PCR Primer, Index 47 (95% over 21bp)
TCTTATACACATCTCCGAGCCCACGAGACAATGGTAGATCTCGTATGCCG4040.6891845786421017No Hit
CATATACACATCTCCGAGCCCACGAGACAATGGTAGATCTCGTATGCCGT2480.4230638007505971No Hit
TCTTTATACACATCTCCGAGCCCACGAGACAATGGTAGATCTCGTATGCC2110.3599454111224838No Hit
CTATACACATCTCCGAGCCCACGAGACAATGGTAGATCTCGTATGCCGTC2010.3428863868986694No Hit
CTTTACACATCTCCGAGCCCACGAGACAATGGTAGATCTCGTATGCCGTC1450.24735585124530876No Hit
TCTTAATACACATCTCCGAGCCCACGAGACAATGGTAGATCTCGTATGCC720.12282497441146367No Hit
TTATACACATCTCCGAGCCCACGAGACAATGGTAGATCTCGTATGCCGTC700.1194131695667008No Hit
CTCTTATACACATCTCCGAGCCCACGAGACAATGGTAGATCTCGTATGCC640.10917775503241214No Hit
CTTAATACACATCTCCGAGCCCACGAGACAATGGTAGATCTCGTATGCCG620.10576595018764927No Hit
TATACACATCTCCGAGCCCACGAGACAATGGTAGATCTCGTATGCCGTCT590.10064824292050495No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTGCAT150.002214354570.053
AGCTTGA150.002214354570.055
TCAGCTT150.002214354570.053
CAGCTTG150.002214354570.054
GTCTTCA150.002214354570.049
GAACACC150.002214354570.055
AATGGAA501.2732926E-1162.99999629
GAAGATC601.8189894E-1258.33333233
CTTCAGC252.3722957E-455.99999651
TCTTTAT509.549694E-1055.9999961
AACCGTA200.006919324452.510
TCTTCAG200.006919324452.550
ATGGAAG608.185452E-1152.530
TGGAAGA608.185452E-1152.531
CAACCGT200.006919324452.59
AATGACC200.006919324452.562
GTTTTTC200.006919324452.514
AAGATCT800.052.534
TATCGGT200.006919324452.524
TTCTGCA200.006919324452.552
Produced by FastQC (version 0.11.5)