FastQCFastQC Report
Wed 25 May 2016
SRR1780999_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780999_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences257900
Sequences flagged as poor quality0
Sequence length76
%GC57

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG5102619.785188057386584No Hit
CTTATACACATCTGACGCTGCCGACGAGGCAACCTGTGTAGATCTCGGTG15390.5967429236138038No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCGGGT150.00222406669.991095
GTGTGCG150.00222406669.991092
ACTGAGT252.3890525E-455.9928675
GTCTACT252.3890525E-455.9928671
ATTCTCG200.006944128352.5034940
GTATTCT200.00694945652.493318
TGCGCGT200.00694945652.493315
GTCGGGC200.00694945652.4933110
ACCTTTT352.0821124E-550.00332323
TCGCCGT2500.048.99375552
GCCGTAT2500.047.59393354
ATCTCGG2600.047.11851542
GATCTCG2600.047.11851541
TTGACAG305.874565E-446.6607259
GTCGCCG2850.046.6607251
TCGGTGG2900.045.8651245
TGGTCGC2900.045.85622849
AGATCTC2700.045.37338640
CGCCGTA2700.045.3645953
CTCGGTG2900.044.65814244
Produced by FastQC (version 0.11.5)