FastQCFastQC Report
Wed 25 May 2016
SRR1780999_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780999_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences257900
Sequences flagged as poor quality0
Sequence length76
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACGTTAACAGATCTCGTATGCCGT3167712.282667700659172No Hit
TCTTATACACATCTCCGAGCCCACGAGACGTTAACAGATCTCGTATGCCG39701.5393563396665375No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG27431.0635905389685925No Hit
CATATACACATCTCCGAGCCCACGAGACGTTAACAGATCTCGTATGCCGT20170.7820860798759208No Hit
CTCTTATACACATCTCCGAGCCCACGAGACGTTAACAGATCTCGTATGCC16760.6498642884839084No Hit
ATACACATCTCCGAGCCCACGAGACGTTAACAGATCTCGTATGCCGTCTT13840.5366421093447072Illumina PCR Primer Index 9 (95% over 22bp)
TCTTTATACACATCTCCGAGCCCACGAGACGTTAACAGATCTCGTATGCC7930.3074835207444746No Hit
CTATACACATCTCCGAGCCCACGAGACGTTAACAGATCTCGTATGCCGTC5440.21093447072508725No Hit
CTTTACACATCTCCGAGCCCACGAGACGTTAACAGATCTCGTATGCCGTC3830.1485071733229934No Hit
CTTATACACATCTCCGAGCCCACGAGACGATAACAGATCTCGTATGCCGT2830.10973245443970531No Hit
CTTATACACATCTCCGAGCCCACGAGACGTTAACAGATCTCGTATGACGT2760.10701822411787515No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CAGTTGT150.002222942470.000011
CGCGCCT150.002222942470.0000170
GATGCGC150.002222942470.0000131
TATAAGC150.002222942470.0000131
GCAAACG207.916981E-570.018
CGAATGC550.063.63636841
AGGGGGG42150.061.2811470
CTTTATA1750.060.02
TGACGTC1250.058.80000345
GACGTCA308.361751E-658.33333646
AAAGATC700.055.033
CGTATGA1450.053.10344741
CGTCTTC50600.052.91501648
GCCGTCT50400.052.8472246
TATGCCG51600.052.8391543
GATCTCG52200.052.76819636
ATGCCGT51350.052.755644
GTATGCC52050.052.7185442
CCGTCTT50000.052.7147
AGATCTC52200.052.70114535
Produced by FastQC (version 0.11.5)