FastQCFastQC Report
Wed 25 May 2016
SRR1780998_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780998_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences34523
Sequences flagged as poor quality0
Sequence length76
%GC90

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG2618475.8450887813921No Hit
CTTATACACATCTGACGCTGCCGACGAGGCAACCTGTGTAGATCTCGGTG12163.5222894881673086No Hit
ATACACATCTGACGCTGCCGACGAGGCAACCTGTGTAGATCTCGGTGGTC1630.47214900211453237No Hit
TCTTATACACATCTGACGCTGCCGACGAGGCAACCTGTGTAGATCTCGGT1250.36207745560930393No Hit
CTCTTATACACATCTGACGCTGCCGACGAGGCAACCTGTGTAGATCTCGG790.2288329519450801No Hit
TCTTTATACACATCTGACGCTGCCGACGAGGCAACCTGTGTAGATCTCGG700.20276337514121023No Hit
GGTGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG470.13614112330909828No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTTATA207.820292E-570.02
TGGCGCT150.002206607570.013
TTTATAC150.002206607570.03
AAAAAGG900.062.22222570
CGCTGGC200.00689530352.516
GGCGCTG200.00689530352.514
GCTGGCG200.00689530352.517
TCGCCGA200.00689530352.552
GCGCTGG200.00689530352.515
TCTTTAT200.00689530352.51
GTCGCCG2000.048.99999651
GGTGGTC2000.048.99999647
GTGGTCG2000.048.99999648
GGTCGCC2000.048.99999650
TGGTCGC1950.048.4615449
CGGTGGT1950.048.4615446
TCGCCGT1850.047.297352
CGCCGTA1850.047.297353
TCGGTGG2000.047.24999645
GTATCAT1800.046.66666857
Produced by FastQC (version 0.11.5)