FastQCFastQC Report
Wed 25 May 2016
SRR1780998_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780998_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences34523
Sequences flagged as poor quality0
Sequence length76
%GC51

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACATCACTCGATCTCGTATGCCGT1245536.07739767691104No Hit
TCTTATACACATCTCCGAGCCCACGAGACATCACTCGATCTCGTATGCCG23046.67381166179069No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG20726.001795904179822No Hit
CATATACACATCTCCGAGCCCACGAGACATCACTCGATCTCGTATGCCGT12723.684500188280277No Hit
ATACACATCTCCGAGCCCACGAGACATCACTCGATCTCGTATGCCGTCTT11973.467253714914695RNA PCR Primer, Index 42 (95% over 22bp)
TCTTTATACACATCTCCGAGCCCACGAGACATCACTCGATCTCGTATGCC7472.1637748747212004No Hit
CTATACACATCTCCGAGCCCACGAGACATCACTCGATCTCGTATGCCGTC5211.509138834979579RNA PCR Primer, Index 32 (95% over 21bp)
CTCTTATACACATCTCCGAGCCCACGAGACATCACTCGATCTCGTATGCC5001.4483098224372157No Hit
CTTTACACATCTCCGAGCCCACGAGACATCACTCGATCTCGTATGCCGTC3380.9790574399675579No Hit
CTTTATACACATCTCCGAGCCCACGAGACATCACTCGATCTCGTATGCCG1880.5445644932363931No Hit
CTTAATACACATCTCCGAGCCCACGAGACATCACTCGATCTCGTATGCCG1870.5416678735915187No Hit
TCTTAATACACATCTCCGAGCCCACGAGACATCACTCGATCTCGTATGCC1660.4808388610491556No Hit
TTATACACATCTCCGAGCCCACGAGACATCACTCGATCTCGTATGCCGTC1540.44607942531066247RNA PCR Primer, Index 32 (95% over 21bp)
CTTATACACATCTCCGAGCCCACGAGACATAACTCGATCTCGTATGCCGT1520.4402861860209136No Hit
TATACACATCTCCGAGCCCACGAGACATCACTCGATCTCGTATGCCGTCT1510.43738956637603915RNA PCR Primer, Index 42 (95% over 21bp)
CTTATACACATCTCCGAGCCCACGAGACATCACTCGATCTCGTATGACGT1420.41131998957216925No Hit
CCTTATACACATCTCCGAGCCCACGAGACATCACTCGATCTCGTATGCCG1330.3852504127682994No Hit
CTTATACACATCTCCGAGCCCACGAGACATCACACGATCTCGTATGCCGT1160.33600787880543403No Hit
CTTATACACATCTCCGAGCCCACGAGACATCACTCGATATCGTATGCCGT1030.29835182342206645No Hit
CTTATACACATCTCCGAGCCCACGAGACATCACTAGATCTCGTATGCCGT950.27517886626307103No Hit
CTAATACACATCTCCGAGCCCACGAGACATCACTCGATCTCGTATGCCGT860.2491092894592011No Hit
CTTATACACATCTCCGAGCCCACGAGACATCACTCGATCTCGTATGCCGA860.2491092894592011No Hit
CTTATACACATCTCCGAGCCCACGAGACATCAATCGATCTCGTATGCCGT820.2375228108797034No Hit
CTTATACACATCTCCGAGCCCACGAGACATCACTCGATCTCGTATGCAGT590.17090055904759147No Hit
CTTATACACATCTCCGAGCCCACGAGACATCACTCGCTCTCGTATGCCGT580.16800393940271702No Hit
TCTTATACACATCTCCGAGCCCACGAGACATCACTCGCTCTCGTATGCCG570.1651073197578426No Hit
TCTATACACATCTCCGAGCCCACGAGACATCACTCGATCTCGTATGCCGT540.1564174608232193No Hit
ACTTATACACATCTCCGAGCCCACGAGACATCACTCGATCTCGTATGCCG530.15352084117834486No Hit
CTTATACACATCTCCGAGCCCACGAGACATCACTCGAACTCGTATGCCGT500.1448309822437216No Hit
TCTTATACACATCTCCGAGCCCACGAGACATCACTCGATCTCGTATGCAG470.13614112330909828No Hit
TCTTATACACATCTCCGAGCCCACGAGACATCACTCGATCTCGTATGACG460.13324450366422386No Hit
CATATACACATCTCCGAGCCCACGAGACATCACTCGATCTCGTATGCCGA460.13324450366422386No Hit
CATATACACATCTCCGAGCCCACGAGACATCACTCGATATCGTATGCCGT450.13034788401934944No Hit
ATTATACACATCTCCGAGCCCACGAGACATCACTCGATCTCGTATGCCGT440.127451264374475No Hit
CATATACACATCTCCGAGCCCACGAGACATCACTCGATCTCGTATGACGT400.11586478579497728No Hit
CTTATACACATCTCCGAGCCAACGAGACATCACTCGATCTCGTATGCCGT380.1100715465052284No Hit
CTTATACACATCACCGAGCCCACGAGACATCACTCGATCTCGTATGCCGT370.10717492686035397No Hit
CTTATACACATCTCCGAGACCACGAGACATCACTCGATCTCGTATGCCGT360.10427830721547954No Hit
CTTATACACATCTCCGAGCCCACGAGACATCACTCGATCACGTATGCCGT350.10138168757060512No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATAAACA353.5724952E-970.000014
ATCACGT353.5724952E-970.0000137
TCTTTAT1100.070.000011
CGATCAC353.5724952E-970.0000135
AGCCCAA207.820292E-570.017
GAGGTAT150.002206607570.037
AATCTCC150.002206607570.09
GCCCAAG207.820292E-570.018
CTTTACA500.070.01
CTCCTTG150.002206607570.054
AGAGGGG252.782981E-670.070
TATAAAC207.820292E-570.03
TTATAAA207.820292E-570.02
CCCAAGA207.820292E-570.019
CTTATAA207.820292E-570.01
TCTCCTT150.002206607570.053
AAGGAGG150.002206607570.069
AGGTATG150.002206607570.038
TCTTCTC207.820292E-570.050
TTTACAC500.070.02
Produced by FastQC (version 0.11.5)