FastQCFastQC Report
Wed 25 May 2016
SRR1780997_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780997_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences206629
Sequences flagged as poor quality0
Sequence length76
%GC58

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG4564022.087896665037338No Hit
CTTATACACATCTGACGCTGCCGACGAGGCAACCTGTGTAGATCTCGGTG22101.0695497727811683No Hit
ATACACATCTGACGCTGCCGACGAGGCAACCTGTGTAGATCTCGGTGGTC2930.1418000377488155No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATTAACG150.002220850470.0116318
CCGGCGA150.002222984669.99467521
TGCCGGC252.8340255E-669.99467519
GCGGCCC200.006946096752.49600613
CTACCCG200.006952750552.483310
CTTTATA200.006952750552.48332
CGGTGGT3700.044.45607846
TGGTCGC3800.044.2071649
TGTGACG404.570631E-543.7678638
GATCTCG3600.043.75726341
GATAACG404.5837216E-543.7466744
TAACGTT404.5837216E-543.7466746
ATAACGT404.5837216E-543.7466745
CGATAAC404.5837216E-543.7466743
AGATCTC3800.043.29665840
TCGCCGT3800.043.2861852
GTCGCCG3800.043.2861851
TCGGTGG3800.043.2861845
GCCGTAT3800.043.2861854
ATCTCGG3700.042.56432742