FastQCFastQC Report
Wed 25 May 2016
SRR1780997_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780997_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences206629
Sequences flagged as poor quality0
Sequence length76
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACATAAGTTAATCTCGTATGCCGT2335911.304802326875704No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG37111.795972491760595No Hit
TCTTATACACATCTCCGAGCCCACGAGACATAAGTTAATCTCGTATGCCG33811.636265964603226No Hit
CATATACACATCTCCGAGCCCACGAGACATAAGTTAATCTCGTATGCCGT20550.9945361009345252No Hit
ATACACATCTCCGAGCCCACGAGACATAAGTTAATCTCGTATGCCGTCTT17380.8411210430288101RNA PCR Primer, Index 15 (95% over 21bp)
CTCTTATACACATCTCCGAGCCCACGAGACATAAGTTAATCTCGTATGCC11570.5599407633971998No Hit
TCTTTATACACATCTCCGAGCCCACGAGACATAAGTTAATCTCGTATGCC9680.4684724796616157No Hit
CTATACACATCTCCGAGCCCACGAGACATAAGTTAATCTCGTATGCCGTC7370.35667791065145743No Hit
CTTATACACATCTCCGAGCCCACGAGACATAAGATAATCTCGTATGCCGT5600.27101713699432317No Hit
CTTTACACATCTCCGAGCCCACGAGACATAAGTTAATCTCGTATGCCGTC4850.23472019900401203No Hit
CTTTATACACATCTCCGAGCCCACGAGACATAAGTTAATCTCGTATGCCG3440.1664819555822271No Hit
CTTATACACATCTCCGAGCCCACGAGACATAAGTAAATCTCGTATGCCGT3040.14712358865406117No Hit
CTTATACACATCTCCGAGCCCACGAGACATAAGTTAATCTCGTATGACGT2760.133572731804345No Hit
TATACACATCTCCGAGCCCACGAGACATAAGTTAATCTCGTATGCCGTCT2390.11566624239579149No Hit
TCTTAATACACATCTCCGAGCCCACGAGACATAAGTTAATCTCGTATGCC2340.11324644652977074No Hit
CTTATACACATCTCCGAGCCCACGAGACATAAGTTAATATCGTATGCCGT2250.10889081397093342No Hit
CTTAATACACATCTCCGAGCCCACGAGACATAAGTTAATCTCGTATGCCG2230.10792289562452512No Hit
TTATACACATCTCCGAGCCCACGAGACATAAGTTAATCTCGTATGCCGTC2110.10211538554607534No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCTTATA252.8335617E-669.996621
CGAATGC550.063.63327841
CCGACTT900.062.2192147
TCGAATG453.8380676E-1062.2192140
AACTCGT700.059.99709337
TGCCGAC1050.059.99709345
AGGGGGG31150.058.87355470
CGACTTC1150.057.82328848
GCCGACT1050.056.6639246
CGGTTCA252.387004E-455.9972914
AGAATGC252.387004E-455.9972941
ATATACA6100.054.5055582
AGCTAGA452.6850103E-854.45498755
AAACTCG850.053.5268236
TCTTTAT1900.053.418471
GACTTAG200.00693869252.51016653
GCATGCA200.00693869252.51016656
CGTCTAC608.367351E-1152.49745648
GCCGACA200.00694533952.49745646
GTCCGTA200.00694533952.49745613