FastQCFastQC Report
Wed 25 May 2016
SRR1780996_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780996_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences125187
Sequences flagged as poor quality0
Sequence length76
%GC73

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG5866746.863492215645394No Hit
CTTATACACATCTGACGCTGCCGACGAGGCAACCTGTGTAGATCTCGGTG16291.3012533250257614No Hit
ATACACATCTGACGCTGCCGACGAGGCAACCTGTGTAGATCTCGGTGGTC1900.15177294767028526No Hit
TCTTATACACATCTGACGCTGCCGACGAGGCAACCTGTGTAGATCTCGGT1620.12940640801361164No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGAGAG150.002222323869.983625
GATCGTA150.002222323869.9836247
ATCGTAT150.002222323869.9836248
CAGATCG150.002222323869.9836245
CACTTGA150.002222323869.9836210
ATGAGCA200.00691119352.550770
ACTACAT200.00691119352.550769
AACGTCT200.00692213852.52968667
AAAAGAT200.00693309652.5086965
TAACTAG200.00693309652.5086929
ATTACCT200.00694406752.48771751
GCCATAG200.00694406752.4877171
TTTCAGT200.00694406752.4877171
CCACTTG200.00694406752.4877179
AGTGCCA200.00694406752.4877175
ATCCCTA200.00694406752.48771711
CAGTGCC200.00694406752.4877174
TCGTATC200.00694406752.48771749
ACCGTTA200.00694406752.48771713
ATAGTGA200.00694406752.4877178
Produced by FastQC (version 0.11.5)