FastQCFastQC Report
Wed 25 May 2016
SRR1780996_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780996_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences125187
Sequences flagged as poor quality0
Sequence length76
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTTGCTAAGATCTCGTATGCCGT3671629.328923929801014No Hit
TCTTATACACATCTCCGAGCCCACGAGACTTGCTAAGATCTCGTATGCCG43473.472405281698579No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG27752.216683840973903No Hit
CATATACACATCTCCGAGCCCACGAGACTTGCTAAGATCTCGTATGCCGT23751.8971618458785657No Hit
CTCTTATACACATCTCCGAGCCCACGAGACTTGCTAAGATCTCGTATGCC21161.6902713540543346No Hit
ATACACATCTCCGAGCCCACGAGACTTGCTAAGATCTCGTATGCCGTCTT18141.449032247757355Illumina PCR Primer Index 9 (95% over 21bp)
TCTTTATACACATCTCCGAGCCCACGAGACTTGCTAAGATCTCGTATGCC9750.7788348630448848No Hit
CTATACACATCTCCGAGCCCACGAGACTTGCTAAGATCTCGTATGCCGTC6950.5551694664781487No Hit
CTTTACACATCTCCGAGCCCACGAGACTTGCTAAGATCTCGTATGCCGTC4840.3866216140653582No Hit
CTTTATACACATCTCCGAGCCCACGAGACTTGCTAAGATCTCGTATGCCG3090.24683074121114812No Hit
CTTATACACATCTCCGAGCCCACGAGACTTGCTAAGATCTCGTATGACGT3010.24044030130924138No Hit
CTTATACACATCTCCGAGCCCACGAGACTTGCTAAGCTCTCGTATGCCGT2700.2156773466893527No Hit
CTTAATACACATCTCCGAGCCCACGAGACTTGCTAAGATCTCGTATGCCG2550.20369527187327757No Hit
CTTATACACATCTCCGAGCCCACGAGACTAGCTAAGATCTCGTATGCCGT2470.19730483197137083No Hit
TCTTAATACACATCTCCGAGCCCACGAGACTTGCTAAGATCTCGTATGCC2320.18532275715529567No Hit
TATACACATCTCCGAGCCCACGAGACTTGCTAAGATCTCGTATGCCGTCT2300.183725147179819No Hit
CTTATACACATCTCCGAGCCCACGAGACATGCTAAGATCTCGTATGCCGT2030.16215741251088372No Hit
CTAATACACATCTCCGAGCCCACGAGACTTGCTAAGATCTCGTATGCCGT1950.15576697260897698No Hit
TTATACACATCTCCGAGCCCACGAGACTTGCTAAGATCTCGTATGCCGTC1850.14777892273159354No Hit
CTTATACACATCTCCGAGCCCACGAGACTTGCTAAGATCTCGTATGCAGT1770.1413884828296868No Hit
CCTTATACACATCTCCGAGCCCACGAGACTTGCTAAGATCTCGTATGCCG1530.12221716312396655No Hit
ACTTATACACATCTCCGAGCCCACGAGACTTGCTAAGATCTCGTATGCCG1260.10064942845503128No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCGGG150.002217296570.02357569
GCGCATA150.002220815369.99560514
TGTGACC150.002220815369.9956054
GTGTGAC150.002220815369.9956053
TGACCGC150.002220815369.9956056
TGCGCAT150.002220815369.99560513
TCTACAG150.002220815369.99560550
TACGATC150.002220815369.99560546
TTGCGCA150.002220815369.99560512
AGTTAAC150.002220815369.99560557
ATACGAT150.002220815369.99560545
GTGACGG150.002220815369.9956059
TAAGACG150.002220815369.99560543
GCCGTAT150.002220815369.99560546
AGAGACT252.8272716E-669.995623
TCTTTAT1800.066.1069641
ATATCGT453.8016879E-1062.24318337
AGGGGGG46900.061.81186370
AAAAGCG352.947545E-760.02021867
ATCGTAT650.059.2507239
Produced by FastQC (version 0.11.5)