FastQCFastQC Report
Wed 25 May 2016
SRR1780995_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780995_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences369463
Sequences flagged as poor quality0
Sequence length76
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG278667.542297875565347No Hit
CTTATACACATCTGACGCTGCCGACGAGGCAACCTGTGTAGATCTCGGTG12370.3348102516354818No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTGCG150.002226658469.9765853
AGCGTGC200.006942635452.51086452
AACGTGA200.006957520352.4824410
GGTCACG200.006957520352.4824411
GTTGCGC200.006957520352.482444
GCCGTAT2350.040.2210954
CAGTAGT350.001255056439.9974481
TCGCCGT2500.037.80782352
TCGGTGG2750.035.62926545
GTCGCCG2700.035.00724451
GCATATA601.2148474E-534.9977651
TGGTCGC2850.034.38842449
CGGTGGT2850.034.37911646
CGCCGTA2900.033.800153
CTCGGTG2900.033.78637344
TCTCGGT2900.033.78637343
GTAGATC3050.032.137838
GATCTCG3050.032.12909741
CGTATCA3000.031.4979956
CGTGCGG450.00430035431.1175554
Produced by FastQC (version 0.11.5)