FastQCFastQC Report
Wed 25 May 2016
SRR1780995_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780995_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences369463
Sequences flagged as poor quality0
Sequence length76
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACATTGCCGGATCTCGTATGCCGT115383.1229108192159973No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG22920.6203598195218466No Hit
TCTTATACACATCTCCGAGCCCACGAGACATTGCCGGATCTCGTATGCCG20880.565144547627232No Hit
ATACACATCTCCGAGCCCACGAGACATTGCCGGATCTCGTATGCCGTCTT9660.26146055220685155RNA PCR Primer, Index 30 (95% over 22bp)
CATATACACATCTCCGAGCCCACGAGACATTGCCGGATCTCGTATGCCGT9650.26118988910932894No Hit
TCTTTATACACATCTCCGAGCCCACGAGACATTGCCGGATCTCGTATGCC6030.16320984780614026No Hit
CTATACACATCTCCGAGCCCACGAGACATTGCCGGATCTCGTATGCCGTC4970.13451955946874247No Hit
CTCTTATACACATCTCCGAGCCCACGAGACATTGCCGGATCTCGTATGCC4760.12883563442076743No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTCTTA1300.059.23798848
AGGGGGG18100.054.71560770
TGACGTA200.006944974452.50639745
CGTGGCA200.006948693752.4992915
GCGTAGA200.006948693752.4992918
CGTAGAG200.006952414352.49218419
CCGTCTT20000.047.605847
GCCGTCT20150.047.5988546
CTCGTAT24550.046.90999239
TCTTCTG18900.046.84487550
ATCTCGT24600.046.81464837
CGTATGC23750.046.7214841
TATGCCG23700.046.6723543
GACGACT305.8706687E-446.67234846
TCTCGTA24750.046.53092238
TCGTATG24800.046.43710740
GATCTCG25450.046.35142536
GTCTTCT19500.045.95431549
TGCCGTC23200.045.86765345
CGTCTTC20000.045.85558748