FastQCFastQC Report
Wed 25 May 2016
SRR1780994_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780994_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences544641
Sequences flagged as poor quality0
Sequence length76
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG6134411.263199061400078No Hit
CTTATACACATCTGACGCTGCCGACGAGGCAACCTGTGTAGATCTCGGTG47750.8767243009615509No Hit
ATACACATCTGACGCTGCCGACGAGGCAACCTGTGTAGATCTCGGTGGTC7440.13660374448489923No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGACGG150.002224215870.0004616
CACGTTA150.002225025869.9940354
CGACAAG200.006944855752.50998768
GACGGAA200.006947378652.50516518
TCCGCAA200.00695242652.4955249
TAAGACG200.00695242652.4955259
GCCGTAT7900.049.1730254
GTCGCCG7850.048.5945851
TCGCCGT7900.047.8440252
TGGTCGC7900.047.8440249
CGGTGGT8050.047.38726446
CTCGGTG8000.047.2459744
TCGGTGG8100.046.6626945
TCTCGGT8200.046.52042443
CGCCGTA8350.046.10385553
GATCTCG8400.045.00029841
ATCTCGG8450.044.7299142
AGATCTC8350.044.43142740
CGTATCA8600.044.3566856
GTAGATC8450.044.32388738
Produced by FastQC (version 0.11.5)