FastQCFastQC Report
Wed 25 May 2016
SRR1780994_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780994_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences544641
Sequences flagged as poor quality0
Sequence length76
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACAGTAGATTATCTCGTATGCCGT366326.725898344046813No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG79821.4655525382775076No Hit
TCTTATACACATCTCCGAGCCCACGAGACAGTAGATTATCTCGTATGCCG42520.780697744018537No Hit
CATATACACATCTCCGAGCCCACGAGACAGTAGATTATCTCGTATGCCGT25110.4610376376365349No Hit
CTCTTATACACATCTCCGAGCCCACGAGACAGTAGATTATCTCGTATGCC20080.36868322436247No Hit
ATACACATCTCCGAGCCCACGAGACAGTAGATTATCTCGTATGCCGTCTT16540.3036862814220744Illumina PCR Primer Index 10 (95% over 23bp)
TCTTTATACACATCTCCGAGCCCACGAGACAGTAGATTATCTCGTATGCC10600.1946236144542919No Hit
CTATACACATCTCCGAGCCCACGAGACAGTAGATTATCTCGTATGCCGTC6590.12099713389186638Illumina PCR Primer Index 10 (95% over 21bp)
CTTATACACATCTCCGAGCCCACGAGACAGTAGATTATCTCGTATGACGT5800.10649216639951821No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGGGGGG49350.060.0041770
AAGCCGT700.059.99865744
GCCGACA509.804353E-1055.99875346
GCCGTCT55850.052.82782746
CCGTCTT55350.052.7359547
CGTATGC59800.052.5573641
TGCCGAC1400.052.49882545
TATGCCG59300.052.41029743
CGTCTTC56400.052.31266448
CTCGTAT62000.052.2778339
TCGTATG61850.052.1782440
CTTGAAA58400.052.01938657
GTATGCC59750.052.01557542
CTGCTTG54650.051.93845754
TCTCGTA62550.051.9300738
ATCTCGT62650.051.7913137
TGCCGTC58450.051.7353745
TCTTCTG55900.051.65358750
ATGCCGT59100.051.52169444
GTCTTCT55950.051.4823249