FastQCFastQC Report
Wed 25 May 2016
SRR1780993_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780993_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences237955
Sequences flagged as poor quality0
Sequence length76
%GC62

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG6678928.0679120001681No Hit
CTTATACACATCTGACGCTGCCGACGAGGCAACCTGTGTAGATCTCGGTG18320.7698934672522115No Hit
ATACACATCTGACGCTGCCGACGAGGCAACCTGTGTAGATCTCGGTGGTC2700.11346683196402682No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACTATCG150.002223101469.9970637
TAGCGCG150.002223101469.9970632
CTCGGTC150.002223101469.9970637
GCGTGTG352.9667353E-759.997488
TGCGCGT308.361854E-658.3308835
TACTTGG252.3851897E-456.00941513
GCTGATA252.3876685E-455.9976438
CACGGCG200.00694068152.50882312
GGTACCG200.00694645352.4977921
CGCTCTA200.00694645352.4977947
GCTAGCG200.00694645352.4977930
CTTTCGC200.00694645352.4977943
TCGAGTA200.00694645352.4977941
CGCGTGT200.00694645352.497797
TGACTTA200.00694645352.497797
CTAGCGC200.00694645352.4977931
CGACGTT305.8650936E-446.6745114
GTTACTC305.8650936E-446.6745118
CGTTACT305.8650936E-446.6745117
GAGCGGA305.871174E-446.66470769
Produced by FastQC (version 0.11.5)