FastQCFastQC Report
Wed 25 May 2016
SRR1780993_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780993_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences237955
Sequences flagged as poor quality0
Sequence length76
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACGTGATAGCATCTCGTATGCCGT4394918.469458511063017No Hit
TCTTATACACATCTCCGAGCCCACGAGACGTGATAGCATCTCGTATGCCG50082.104599609169801No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG32771.3771511420226514No Hit
CTCTTATACACATCTCCGAGCCCACGAGACGTGATAGCATCTCGTATGCC24851.0443151015948393No Hit
CATATACACATCTCCGAGCCCACGAGACGTGATAGCATCTCGTATGCCGT22160.9312685171566052No Hit
ATACACATCTCCGAGCCCACGAGACGTGATAGCATCTCGTATGCCGTCTT15870.666932823433002RNA PCR Primer, Index 24 (95% over 23bp)
TCTTTATACACATCTCCGAGCCCACGAGACGTGATAGCATCTCGTATGCC10880.45722930806244877No Hit
CTATACACATCTCCGAGCCCACGAGACGTGATAGCATCTCGTATGCCGTC6540.2748418818684205No Hit
CTTATACACATCTCCGAGCCCACGAGACGTGATAGCATCTCGTATGACGT4830.2029795549578702No Hit
CTTTACACATCTCCGAGCCCACGAGACGTGATAGCATCTCGTATGCCGTC4300.18070643609085751RNA PCR Primer, Index 24 (95% over 21bp)
CTTTATACACATCTCCGAGCCCACGAGACGTGATAGCATCTCGTATGCCG3990.16767876279128408No Hit
CTTATACACATCTCCGAGCCCACGAGACGAGATAGCATCTCGTATGCCGT2550.10716311907713644No Hit
CTTATACACATCTCCGAGCCCACGAGACGTGATAGCATCTCGTATGCAGT2420.10169990124183144No Hit
CTTAATACACATCTCCGAGCCCACGAGACGTGATAGCATCTCGTATGCCG2420.10169990124183144No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTAAGC252.834171E-669.9995851
CGCGCTA150.002222783669.9995822
CAACACG150.002222783669.9995836
CGCTAGT150.002222783669.9995824
GTATCCC207.915958E-569.999586
GCGCTAG150.002222783669.9995823
GCCAAGA150.002222783669.9995833
AGGGGGG51350.064.7513170
CACGTAT453.8380676E-1062.2218539
GAATCTC750.055.99966435
AATCTCG750.055.99966436
CGAATGC750.055.99966441
GCCGTAA252.3872431E-455.99966446
ACGAGAT1400.054.99967227
TCTTTAT2300.054.7937931
ACTCCGA452.6915586E-854.4441211
TGCCGAC900.054.4441245
CTTTATA2450.052.8679352
CCGTCTT61200.052.78563347
CGTCTTC62400.052.72404548
Produced by FastQC (version 0.11.5)