FastQCFastQC Report
Wed 25 May 2016
SRR1780992_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780992_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences721455
Sequences flagged as poor quality0
Sequence length76
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG561897.7882889438703735No Hit
CTTATACACATCTGACGCTGCCGACGAGGCAACCTGTGTAGATCTCGGTG21390.2964841882030064No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCGAGT200.006950013352.50187323
CTAACGC305.870884E-446.6780469
CGATCTT305.880923E-446.6618614
GCCGTAT3550.045.35058654
CGCCGTA3900.043.97287853
TCGCCGT3750.042.9318952
TGGTCGC3850.042.72583849
GTCGCCG3850.041.81677651
CGTATCA3950.040.75247656
AGTTCGC350.001256521839.9931158
GTGGTCG4250.037.87845248
CTCGGTG4350.037.812244
TCTCGGT4450.037.75153743
CGGTGGT4300.037.43800446
TCGGTGG4500.036.55179245
GTAGATC4750.036.1090538
ATTGGCG400.002419237734.9988255
CCGTATC4850.033.1947655
GATCTCG4950.032.52866741
GGTCGCC5100.032.25381550
Produced by FastQC (version 0.11.5)