FastQCFastQC Report
Wed 25 May 2016
SRR1780992_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780992_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences721455
Sequences flagged as poor quality0
Sequence length76
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTACTTGAAATCTCGTATGCCGT294514.082167286940973No Hit
TCTTATACACATCTCCGAGCCCACGAGACTACTTGAAATCTCGTATGCCG42060.5829885439840323No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG36480.5056448427136827No Hit
CATATACACATCTCCGAGCCCACGAGACTACTTGAAATCTCGTATGCCGT26230.36357083948409813No Hit
ATACACATCTCCGAGCCCACGAGACTACTTGAAATCTCGTATGCCGTCTT18940.2625250362115447TruSeq Adapter, Index 12 (95% over 23bp)
CTCTTATACACATCTCCGAGCCCACGAGACTACTTGAAATCTCGTATGCC16800.23286275651288021No Hit
TCTTTATACACATCTCCGAGCCCACGAGACTACTTGAAATCTCGTATGCC11350.15732096942983276No Hit
CTATACACATCTCCGAGCCCACGAGACTACTTGAAATCTCGTATGCCGTC8780.12169851203470763TruSeq Adapter, Index 12 (95% over 21bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGGGGGG41450.056.40357670
GCGGTAA252.3908017E-455.9982820
GCCGTCT52500.047.59853746
CGTCTTC52800.047.5932448
CCGTCTT51850.047.58773847
TGCCGTC53950.047.29233645
TATGCCG54200.047.26792543
ATGCCGT54050.047.2048444
TCGTATG56300.046.7481240
CGTATGC55350.046.72846641
AGCGGTA305.878828E-446.66523719
CTCGTAT56150.046.56134839
TCTCGTA56750.046.5007838
GTATGCC55350.046.3490742
ATCTCGT56950.046.2760237
CTGCTTG52650.046.07015254
AATCTCG58250.045.96425636
GTCTTCT54250.045.03087649
TCTGCTT53800.044.69503453
CTTCTGC55350.044.51534351
Produced by FastQC (version 0.11.5)