FastQCFastQC Report
Wed 25 May 2016
SRR1780991_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780991_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences726194
Sequences flagged as poor quality0
Sequence length76
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG602388.295028601172689No Hit
CTTATACACATCTGACGCTGCCGACGAGGCAACCTGTGTAGATCTCGGTG13480.18562532876889645No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGAATT200.006953120752.4959754
TACGCGT200.00695880452.4851265
ACGCGTC350.001257213939.9886676
GCCGTAT2850.039.2952354
TCGGTGG3650.036.43556245
TCGCCGT2850.035.611352
CGGTGGT3300.034.99731446
GTACTAG904.5110937E-934.9949041
GATCTTA501.7113282E-434.9949041
CTCGGTG3650.033.5590744
GATCTCG3450.033.48030541
TGGTCGC3350.033.42566749
CGCGTCT851.06674634E-732.9318437
GTCGCCG3250.032.3052151
GGGTCTA652.1050548E-532.3029861
GGTCTAA652.1050548E-532.3029861
ATCTCGG3700.031.2181242
GTAGATC3850.030.00389938
GATATTA703.4925615E-529.995631
TCTCGGT4250.029.64478343
Produced by FastQC (version 0.11.5)