FastQCFastQC Report
Wed 25 May 2016
SRR1780991_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780991_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences726194
Sequences flagged as poor quality0
Sequence length76
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACACAAAGTGATCTCGTATGCCGT373375.141463575848878TruSeq Adapter, Index 5 (95% over 23bp)
TCTTATACACATCTCCGAGCCCACGAGACACAAAGTGATCTCGTATGCCG43460.598462669754914TruSeq Adapter, Index 5 (95% over 22bp)
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG25180.3467393010683095No Hit
CTCTTATACACATCTCCGAGCCCACGAGACACAAAGTGATCTCGTATGCC23400.3222279445988262RNA PCR Primer, Index 5 (95% over 21bp)
CATATACACATCTCCGAGCCCACGAGACACAAAGTGATCTCGTATGCCGT20380.2806412611506016TruSeq Adapter, Index 5 (95% over 23bp)
ATACACATCTCCGAGCCCACGAGACACAAAGTGATCTCGTATGCCGTCTT16860.23216936521094916TruSeq Adapter, Index 5 (96% over 26bp)
TCTTTATACACATCTCCGAGCCCACGAGACACAAAGTGATCTCGTATGCC9710.13371082658353003RNA PCR Primer, Index 5 (95% over 21bp)

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGATCGG150.002224434670.001112
AGGGGGG49350.058.7952570
GACGTGT200.006950579652.50082415
CCTATCG200.006952472552.497212
CCGTCTT55000.051.42253547
TATGCCG58600.051.36599743
GCCGTCT55450.051.32084346
CGTATGC59250.051.04229441
CGTCTTC55850.050.9532848
AACTCGT552.2828317E-950.913437
TCGTATG60450.050.55018640
CTCGTAT59850.050.53059439
ATGCCGT59050.050.50037444
ATCTCGT59550.050.49125737
TGCCGTC58400.050.4031945
GTCTTCT55600.050.3639749
GTATGCC59950.050.3295342
TCTCGTA60250.050.25321238
TCTTCTG56100.050.0988350
CTGCTTG56250.050.0862354
Produced by FastQC (version 0.11.5)